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  1. B100 理学部/理学研究科
  2. B100d 学位論文
  3. 博士論文(理博・論理博)

The tyrosinase gene from medakafish,Oryzias latipes

http://hdl.handle.net/2237/11497
http://hdl.handle.net/2237/11497
cb46c0c8-cf99-4f33-9a34-5fe15c6f8517
名前 / ファイル ライセンス アクション
ko4141.pdf ko4141.pdf (1.7 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2009-04-17
タイトル
タイトル The tyrosinase gene from medakafish,Oryzias latipes
言語 en
その他のタイトル
その他のタイトル メダカOryzias latipesのチロシナーゼ遺伝子の解析
言語 ja
著者 稲垣, 秀人

× 稲垣, 秀人

WEKO 29342

ja 稲垣, 秀人

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
抄録
内容記述 I have determined the 9.8kb genomic nucleotide sequence of the tyrosinase gene and its 5’ upstream region from a teleost, medakafish (Oryzias latipes), and shown that the coding region is composed of five exons and four introns, spanning 4.7kb. While the number and sizes of the exons were found to be similar to those of mammalian tyrosinase genes, however, the total size of the coding region (4.7kb) was demonstrated to be less than one tenth those of mouse (ca. 70kb) and human (>70kb) genes. Primer extension analysis revealed that the transcription initiation site starts with a long untranslated leader sequence (340 nucleotide long) from the AUG start codon. A characteristic CATGTG sequence known as a putative regulatory motif in melanocyte-specific genes was present in the 131th base upstream from the initiation site, while other typical regulatory elements such as the TATA-box or M-box common to terrestrial vertebrates were lacking. Transgenic experiments were carried out by microinjecting two kinds of plasmid clones into fertilized eggs of the albino i^1 mutant; one consisting of the genomic tyrosinase gene with the 10kb 5’ upstream region, and the other the tyrosinase cDNA with the 3kb 5’ upstream region. The results showed that 53 and 45 of 114 and 118 transgenic eggs, respectively, developed normally beyond hatching and 15 and 10 exhibited a mosaic pattern of pigmentation. Despite the absence of typical regulatory elements like a TATA-box in both cases correct melanin pigmentation was obtained without ectopic expression. Thus, transgenic expression rescued from the albino-i^1 mutation, and the i locus of the medaka genome can be concluded to encode the tyrosinase gene.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 名古屋大学博士学位論文 学位の種類:博士(理学) (課程) 学位授与年月日:平成10年9月30日
言語 ja
内容記述タイプ Other
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
書誌情報
発行日 1998-09-30
学位名
言語 ja
学位名 博士(理学)
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 13901
言語 ja
学位授与機関名 名古屋大学
言語 en
学位授与機関名 Nagoya University
学位授与年度
学位授与年度 1998
学位授与年月日
学位授与年月日 1998-09-30
学位授与番号
学位授与番号 甲第4141号
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/11497
識別子タイプ HDL
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