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The Initial Report of the Establishment of Primary Liver Cell Cultures from Medaka (Oryzias latipes)
https://doi.org/10.18999/fisbjm.8.47
https://doi.org/10.18999/fisbjm.8.478e105645-87b2-4c4b-8282-f90bbd375b02
名前 / ファイル | ライセンス | アクション |
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KJ00005673908.pdf (991.9 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2010-06-21 | |||||
タイトル | ||||||
タイトル | The Initial Report of the Establishment of Primary Liver Cell Cultures from Medaka (Oryzias latipes) | |||||
言語 | en | |||||
著者 |
Cao, Yu-An
× Cao, Yu-An× Blair, James B.× Ostrander, Gary K. |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University | |||||
権利 | ||||||
言語 | ja | |||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
抄録 | ||||||
内容記述 | We report on the establishment of primary liver cell cultures from medaka, Oryzias latipes. We utilized serum-free media, positively-charged Primaria dishes, and collagenase for disassociation of medaka liver cells to establish initial culture conditions. Nearly homogenous (about 96%) primary hepatocyte cultures were initiated and remained viable and proliferative for 30 days. Cultured cells exhibited either a cuboidal/epithelial-like phenotype or spindle-shaped phenotype. Both cell types appeared to have been derived from liver epithelia, as verified by western blotting of cytokeratins and transmission electron microscopy. The predominate cell type was the spindle cell which proliferated to confluence in primary culture. The initial behavior, proliferation characteristics, and media requirements for attachment and spreading of these cells are described. Primary cultures of medaka liver cells have the potential to serve as a useful model for a variety of investigations including, but not limited to, liver cell development, function, and xenobiotic metabolism in an established fish model. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | Regular Paper | |||||
言語 | en | |||||
内容記述タイプ | Other | |||||
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言語 | en | |||||
出版者 | Laboratory of Freshwater Fish Stocks Bioscience Center Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/fisbjm.8.47 | |||||
ID登録タイプ | JaLC | |||||
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関連タイプ | isVersionOf | |||||
識別子タイプ | URI | |||||
関連識別子 | http://ci.nii.ac.jp/naid/110007349027/ | |||||
書誌情報 |
en : The Fish Biology Journal Medaka 巻 8, p. 47-56, 発行日 1996 |
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application/pdf | ||||||
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値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/13815 | |||||
識別子タイプ | HDL |