{"_buckets": {"deposit": "3be1e1b0-193f-4d73-bc2f-652ca2cc64b9"}, "_deposit": {"id": "1368", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1368"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00001368"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1/2", "bibliographicPageEnd": "42", "bibliographicPageStart": "40", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "We identified ZAKI-4 as a thyroid hormone (T3) responsive gene in human skin fibroblasts. Our recent study revealed that two ZAKI-4 isoforms, \u03b1 and \u03b2, are generated from a single gene on chromosome 6. Only expression of \u03b1 is increased by T3, while that of \u03b2 is not affected. Both isoforms belong to a family of proteins containing a conserved motif, which interacts with calcineurin and inhibits its activity. Recently, expression of a mouse homologue of the ZAKI-4 family gene (MCIP1) is shown to be induced by the activation of calcineurin in myocytes. We thus studied whether ZAKI-4 expression is also regulated by calcineurin-mediated pathway. Northern blot analysis revealed activation of calcineurin by ionomycin/phorbol 12-myristate 13-acetate (PMA) did not increase the expression of both isoforms. In addition, either basal expression of the two isoforms or T3-induced ZAKI-4\u03b1 expression was not affected by FK506, a specific calcineurin inhibitor, suggesting the activation of calcineurin is not involved in ZAKI-4 regulation. However, cyclosporin A (CsA), another inhibitor of calcineurin decreased the basal expression of ZAKI-4\u03b1. Furthermore, T3-mediated increase of ZAKI-4\u03b1 was completely blocked by CsA, but not by FK506. It is thus indicated that the two isoforms are differentially regulated, and the regulation of ZAKI-4\u03b1 involves a CsA-sensitive pathway, which is independent of calcineurin activation.", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u56fd\u7acb\u60c5\u5831\u5b66\u7814\u7a76\u6240\u3067\u96fb\u5b50\u5316\u3057\u305f\u30b3\u30f3\u30c6\u30f3\u30c4\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u3002", "subitem_description_type": "Other"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/2778"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/envm.46.40", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Research Institute of Environmental Medicine, Nagoya University"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "02870517", "subitem_source_identifier_type": "ISSN"}]}, "item_9_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "CAO, Xia"}], "nameIdentifiers": [{"nameIdentifier": "3103", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KAMBE, Fukushi"}], "nameIdentifiers": [{"nameIdentifier": "3104", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SEO, Hisao"}], "nameIdentifiers": [{"nameIdentifier": "3105", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000034858.pdf", "filesize": [{"value": "549.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 549600.0, "url": {"label": "KJ00000034858.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/1368/files/KJ00000034858.pdf"}, "version_id": "bd3939d0-18d7-401d-a334-fc6444c94cdf"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "ZAKI-4", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcineurin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CsA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "FK506", "subitem_subject_scheme": "Other"}, {"subitem_subject": "thyroid hormone", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone"}]}, "item_type_id": "9", "owner": "1", "path": ["329/333/334/335"], "permalink_uri": "https://doi.org/10.18999/envm.46.40", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2006-01-05"}, "publish_date": "2006-01-05", "publish_status": "0", "recid": "1368", "relation": {}, "relation_version_is_last": true, "title": ["Cyclosporin A (CsA)-sensitive Pathway for the Induction of ZAKI-4 Expression by Thyroid Hormone"], "weko_shared_id": null}