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Optimal Population of Embryonic Stem Cells in "Hanging Drop" Culture for in-vitro Differentiation to Cardiac Myocytes
https://doi.org/10.18999/envm.46.92
https://doi.org/10.18999/envm.46.92d57e3704-6f2e-459d-acfc-bac446808187
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000034875.pdf (272.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-01-05 | |||||
| タイトル | ||||||
| タイトル | Optimal Population of Embryonic Stem Cells in "Hanging Drop" Culture for in-vitro Differentiation to Cardiac Myocytes | |||||
| 言語 | en | |||||
| 著者 |
MIWA, Keiko
× MIWA, Keiko× LEE, Jong-Kook× HIDAKA, Kyoko× SHI, Rong-qian× MORISAKI, Takayuki× KODAMA, Itsuo |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | embryonic stem cell | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | fluorescence-activated cell sorting | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Pluripotent embryonic stem (ES) cells differentiate to cardiac myocytes in vitro by many other previous reports demonstrated "hanging-drop" method. In this study, the number of ES cells in each hanging-drop plays an important role in the cultivation of cardiac myocytes. We examined the optimal hanging-drop size to obtain embryonic stem cell-derived cardiac cells (ESCMs) in vitro using specific labeled mouse ES cells (hCGP7) which were stably transfected with the enhanced green fluorescent protein (GFP) under transcriptional control of the cardiac myocyte-specific promoter with fluorescence-activated cell sorting (FACS) analysis. GFP-positive cells were sorted efficiently in the hanging-drop cell number of approximately 800～1600 cells/drop. The present data will be an important element to establish the optimal methods of ES cellular differentiation in vitro. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 国立情報学研究所で電子化したコンテンツを使用している。 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Research Institute of Environmental Medicine, Nagoya University | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/envm.46.92 | |||||
| ID登録タイプ | JaLC | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 02870517 | |||||
| 書誌情報 |
en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 巻 46, 号 1/2, p. 92-94, 発行日 2002-12 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/2795 | |||||
| 識別子タイプ | HDL | |||||
