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Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin β -subunit by disruption of disulfide bonds
http://hdl.handle.net/2237/16683
http://hdl.handle.net/2237/16683f6374039-effe-4c03-a854-8fd4ad216601
名前 / ファイル | ライセンス | アクション |
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o5164.pdf (1.7 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2012-07-27 | |||||
タイトル | ||||||
タイトル | Alteration of N-linked oligosaccharide structures of human chorionic gonadotropin β -subunit by disruption of disulfide bonds | |||||
言語 | en | |||||
著者 |
MORIWAKI, TAKAYUKI
× MORIWAKI, TAKAYUKI× SUGANUMA, NOBUHIKO× FURUHASHI, MADOKA× KIKKAWA, FUMITAKA× TOMODA, YUTAKA× BOIME, IRVING× NAKATA, MUNEHIRO× MIZUOCHI, TSUGUO |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | The final publication is available at www.springerlink.com | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human chorionic gonadotropin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | hCG β-subunit | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | disulfide bond | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | N-linked oligosaccharide processing | |||||
抄録 | ||||||
内容記述 | The human chorionic gonadotropin β-subunit (hCGβ) is a glycoprotein in which 12 cysteine residues pair to form six intramolecular disulfide bonds. In order to elucidate the effect of each disulfide bond on glycosylation of the molecule, we analysed structures of asparagine-linked oligosaccharides of various recombinant hCGβ produced in Chinese hamster ovary (CHO) cells: wild-type hCGβ (βWT) and mutants in which any one of the six intramolecular disulfide bonds had been disrupted by site-directed mutagenesis. SDS-PAGE analysis of βWT and these mutants before and after digestion with endoglycosidase F and H revealed structural changes in the oligosaccharide moieties of some mutants. In addition, structural analysis of oligosaccharides obtained from metabolically labeled βWT and a mutant showed that the mutant contained additional high mannose type oligosaccharides. These results suggest that elimination of a specific disulfide bond, resulting in a change in the protein conformation, disturbs the normal assembly of the mature complex type oligosaccharides in the hCGβ molecule. Abbreviations: hCGβ, human chorionic gonadotropin β-subunit; βWT, wild type hCGβ; CHO, Chinese hamster ovary; Endo-H, endoglycosidase H; Endo-F, endoglycosidase F | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 名古屋大学博士学位論文 学位の種類 : 博士(医学)(論文) 学位授与年月日:平成9年2月28日 森脇崇之氏の博士論文として提出された | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Springer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_db06 | |||||
タイプ | doctoral thesis | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1023/A:1018593805890 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0282-0080 | |||||
書誌情報 |
en : Glycoconjugate Journal 巻 14, 号 2, p. 225-229, 発行日 1997-02 |
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学位名 | ||||||
言語 | ja | |||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 13901 | |||||
言語 | ja | |||||
学位授与機関名 | 名古屋大学 | |||||
言語 | en | |||||
学位授与機関名 | NAGOYA University | |||||
学位授与年度 | ||||||
学位授与年度 | 1996 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1997-02-01 | |||||
学位授与番号 | ||||||
学位授与番号 | 乙第5164号 | |||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://dx.doi.org/10.1023/A:1018593805890 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/16683 | |||||
識別子タイプ | HDL |