ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "c4052d46-b8b0-4f19-8d54-9f11b3b7e7d6"}, "_deposit": {"id": "15587", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "15587"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00015587", "sets": ["1468"]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "111", "bibliographicPageStart": "101", "bibliographicVolumeNumber": "75", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Autologous chondrocyte implantation (ACI) is a method of cartilage repair. To improve the quality of regenerated tissue by ACI, it is essential to identify surface marker expression correlated with the differentiation status of monolayer expanded human articular chondrocytes and to define the index for discriminating dedifferentiated cells from monolayer expanded human articular chondrocytes. Normal human articular chondrocytes were cultured in monolayer until passage 4. At each passage, mRNA expression of collagen type I, II, and X and aggrecan was analyzed by real-time quantitative PCR, and the surface marker expression of CD14, CD26, CD44, CD49a, CD49c, CD54, and CD151 was analyzed by fluorescenceactivated cell sorting (FACS). The ratios of mRNA levels of collagen type II to I (Col II/Col I) represented the differentiation status of chondrocytes more appropriately during monolayer culture. The surface marker expression of CD44, CD49c, and CD151 was upregulated according to the dedifferentiation status, whereas that of CD14, CD49a, and CD54 was downregulated. The most appropriate combination of the ratio of Col II/Col I was CD54 and CD44. Cell sorting was performed using a magnetic cell sorting system (MACS) according to CD54 and CD44, and real-time quantitative PCR was performed for the cell subpopulations before and after cell sorting. The expression of collagen type II and aggrecan of the chondrocytes after MACS was higher than that before sorting, but not significantly. The mean fluorescence intensity (MFI) ratio of CD54 to CD44 could be an adequate candidate as the index of the differentiation status.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7512/7512.html"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/17606"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.75.1-2.101", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine"}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN(print)", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "HAMADA, TAKASHI"}], "nameIdentifiers": [{"nameIdentifier": "47495", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SAKAI, TADAHIRO"}], "nameIdentifiers": [{"nameIdentifier": "47496", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIRAIWA, HIDEKI"}], "nameIdentifiers": [{"nameIdentifier": "47497", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "NAKASHIMA, MOTOSHIGE"}], "nameIdentifiers": [{"nameIdentifier": "47498", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ONO, YOHEI"}], "nameIdentifiers": [{"nameIdentifier": "47499", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MITSUYAMA, HIROHITO"}], "nameIdentifiers": [{"nameIdentifier": "47500", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ISHIGURO, NAOKI"}], "nameIdentifiers": [{"nameIdentifier": "47501", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "11_Hamada.pdf", "filesize": [{"value": "843.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 843100.0, "url": {"label": "11_Hamada.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/15587/files/11_Hamada.pdf"}, "version_id": "0d672873-121f-4378-b0bd-4880923a107d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Autologous chondrocyte implantation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "surface marker", "subitem_subject_scheme": "Other"}, {"subitem_subject": "differentiation index", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CD44", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ICAM-1", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes"}]}, "item_type_id": "9", "owner": "1", "path": ["1468"], "permalink_uri": "https://doi.org/10.18999/nagjms.75.1-2.101", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-03-01"}, "publish_date": "2013-03-01", "publish_status": "0", "recid": "15587", "relation": {}, "relation_version_is_last": true, "title": ["Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes"], "weko_shared_id": null}
  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 75(1-2)

Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes

https://doi.org/10.18999/nagjms.75.1-2.101
https://doi.org/10.18999/nagjms.75.1-2.101
8ce04515-8b79-4f0b-8131-9ed7100c66ce
名前 / ファイル ライセンス アクション
11_Hamada.pdf 11_Hamada.pdf (843.1 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2013-03-01
タイトル
タイトル Surface Markers and Gene Expression to Characterize the Differentiation of Monolayer Expanded Human Articular Chondrocytes
著者 HAMADA, TAKASHI

× HAMADA, TAKASHI

WEKO 47495

HAMADA, TAKASHI

Search repository
SAKAI, TADAHIRO

× SAKAI, TADAHIRO

WEKO 47496

SAKAI, TADAHIRO

Search repository
HIRAIWA, HIDEKI

× HIRAIWA, HIDEKI

WEKO 47497

HIRAIWA, HIDEKI

Search repository
NAKASHIMA, MOTOSHIGE

× NAKASHIMA, MOTOSHIGE

WEKO 47498

NAKASHIMA, MOTOSHIGE

Search repository
ONO, YOHEI

× ONO, YOHEI

WEKO 47499

ONO, YOHEI

Search repository
MITSUYAMA, HIROHITO

× MITSUYAMA, HIROHITO

WEKO 47500

MITSUYAMA, HIROHITO

Search repository
ISHIGURO, NAOKI

× ISHIGURO, NAOKI

WEKO 47501

ISHIGURO, NAOKI

Search repository
キーワード
主題Scheme Other
主題 Autologous chondrocyte implantation
キーワード
主題Scheme Other
主題 surface marker
キーワード
主題Scheme Other
主題 differentiation index
キーワード
主題Scheme Other
主題 CD44
キーワード
主題Scheme Other
主題 ICAM-1
抄録
内容記述 Autologous chondrocyte implantation (ACI) is a method of cartilage repair. To improve the quality of regenerated tissue by ACI, it is essential to identify surface marker expression correlated with the differentiation status of monolayer expanded human articular chondrocytes and to define the index for discriminating dedifferentiated cells from monolayer expanded human articular chondrocytes. Normal human articular chondrocytes were cultured in monolayer until passage 4. At each passage, mRNA expression of collagen type I, II, and X and aggrecan was analyzed by real-time quantitative PCR, and the surface marker expression of CD14, CD26, CD44, CD49a, CD49c, CD54, and CD151 was analyzed by fluorescenceactivated cell sorting (FACS). The ratios of mRNA levels of collagen type II to I (Col II/Col I) represented the differentiation status of chondrocytes more appropriately during monolayer culture. The surface marker expression of CD44, CD49c, and CD151 was upregulated according to the dedifferentiation status, whereas that of CD14, CD49a, and CD54 was downregulated. The most appropriate combination of the ratio of Col II/Col I was CD54 and CD44. Cell sorting was performed using a magnetic cell sorting system (MACS) according to CD54 and CD44, and real-time quantitative PCR was performed for the cell subpopulations before and after cell sorting. The expression of collagen type II and aggrecan of the chondrocytes after MACS was higher than that before sorting, but not significantly. The mean fluorescence intensity (MFI) ratio of CD54 to CD44 could be an adequate candidate as the index of the differentiation status.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.75.1-2.101
ID登録タイプ JaLC
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
書誌情報 Nagoya Journal of Medical Science

巻 75, 号 1-2, p. 101-111, 発行日 2013-02
著者版フラグ
値 publisher
URI
識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/7512/7512.html
識別子タイプ URI
URI
識別子 http://hdl.handle.net/2237/17606
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 17:49:27.259616
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3