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Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR
http://hdl.handle.net/2237/0002003788
http://hdl.handle.net/2237/00020037887ca97e5c-28d9-4763-b989-bf512f850478
| 名前 / ファイル | ライセンス | アクション |
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JMV_Yamaguchi.pdf (524 KB)
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figures_Yamaguchi.pdf (366 KB)
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| Item type | itemtype_ver1(1) | |||||||||||||||||||||
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| 公開日 | 2022-09-14 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 著者 |
Yamaguchi, Makoto
× Yamaguchi, Makoto
× Kawada, Jun‐ichi
× Torii, Yuka
× Haruta, Kazunori
× Suzuki, Takako
× Horiba, Kazuhiro
× Takahashi, Yoshiyuki
× Ito, Yoshinori
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| アクセス権 | ||||||||||||||||||||||
| アクセス権 | open access | |||||||||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 権利情報 | "This is the peer reviewed version of the following article: [Yamaguchi, M, Kawada, J-i, Torii, Y, et al. Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR. J Med Virol. 2022; 94: 4559- 4564. doi:10.1002/jmv.27844], which has been published in final form at [https://doi.org/10.1002/jmv.27844]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited." | |||||||||||||||||||||
| 内容記述 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Congenital cytomegalovirus infection (cCMV) is a common cause of congenital infections, leading to neurodevelopmental sequelae. Real-time quantitative polymerase chain reaction (qPCR) has been widely used for the diagnosis and assessment of cCMV; however, the correlation between CMV DNA load and the severity of cCMV symptoms has been inconclusive. Droplet digital PCR (ddPCR) offers an improvement over the current qPCR methods through the absolute quantification of viral loads. We compared ddPCR and qPCR results for the quantification of CMV DNA in blood and urine specimens from 39 neonates with cCMV (21 symptomatic and 18 asymptomatic). There was no significant difference in blood CMV DNA loads measured by ddPCR and qPCR, with or without any clinical findings. However, developmental delays at 36 months were significantly more frequently observed in patients with high CMV DNA loads (≥2950 copies/ml), as measured by ddPCR at diagnosis, than in those with lower CMV DNA loads. The association of urine CMV DNA load with symptoms and developmental delay was not observed. CMV DNA loads in the blood might be used as a predictor of developmental outcomes in cCMV patients, and absolute quantitation of viral loads by ddPCR assay could contribute to the standardization of CMV load measurement. | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | Wiley | |||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| タイプ | journal article | |||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||
| 関連情報 | ||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1002/jmv.27844 | |||||||||||||||||||||
| 収録物識別子 | ||||||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||||||
| 収録物識別子 | 0146-6615 | |||||||||||||||||||||
| 書誌情報 |
en : Journal of Medical Virology 巻 94, 号 9, p. 4559-4564, 発行日 2022-09 |
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| ファイル公開日 | ||||||||||||||||||||||
| 日付 | 2023-09-01 | |||||||||||||||||||||
| 日付タイプ | Available | |||||||||||||||||||||
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Cite as
Yamaguchi, Makoto, Kawada, Jun‐ichi, Torii, Yuka, Haruta, Kazunori, Suzuki, Takako, Horiba, Kazuhiro, Takahashi, Yoshiyuki, Ito, Yoshinori, 2022, Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR: Wiley, 4559–4564 p.
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