| Item type |
itemtype_ver1(1) |
| 公開日 |
2023-04-11 |
| タイトル |
|
|
タイトル |
ラットC-ペプチドのエンザイイムノアッセイ測定法の確立 |
|
言語 |
ja |
| その他のタイトル |
|
|
その他のタイトル |
Enzyme immunoassay for rat C-peptide |
|
言語 |
en |
| 著者 |
李, 玲
押田, 芳治
羅, 偉権
中尾, 千登世
JOHANSSON, B-L.
WAHREN, J.
矢内原, 昇
佐藤, 祐造
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Our previous studies suggest that C-peptide augments glucose utilization in diabetic rats. However, a reliable assay for rat C-peptide has not been available. In the present study, a sensitive and specific enzyme immunoassay for rat C-peptide has been developed. Effects of rat, pig, goat and fetal bovine sera on the standard curve were studied, and did not produced significant displacement. Rat C-peptide standard concentration ranged from 0.024-100 ng/ml. The intraassy coefficient of variation (CV) was 3.9 ± 2.2% (less than 8%). The fasting and postprandial plasma C-peptide level in normal rat were 6.89 ng/ml and 13.28 ng/ml, respectively. Rat plasma C-peptide levels following oral glucose tolerance test in normal rats were consistent with expected patterns. In conclusion, we have developed and validated a rat C-peptide enzyme immunoassay that is sensitive and simple for rat C-peptide in plasma. This assay provides a reliable tool for studies of diabetes using rat models. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
名古屋大学総合保健体育科学センター |
|
言語 |
ja |
| 出版者 |
|
|
出版者 |
The Research Center of Health, Physical Fitness and Sports, Nagoya University |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
|
|
ID登録 |
10.18999/njhpfs.20.1.85 |
|
ID登録タイプ |
JaLC |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0289-5412 |
| 書誌情報 |
ja : 総合保健体育科学
en : Nagoya Journal of Health, Physical Fitness & Sports
巻 20,
号 1,
p. 85-90,
発行日 1997-03-31
|
njhpfs_20_1_85.pdf (2.7 MB)
