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  1. H110 遺伝子実験施設
  2. H110a 雑誌掲載論文
  3. 学術雑誌

P-class pentatricopeptide repeat protein PTSF1 is required for splicing of the plastid pre-tRNAIle in Physcomitrella patens

http://hdl.handle.net/2237/25167
http://hdl.handle.net/2237/25167
3e702e49-5952-48d8-b82e-7e17f91f136d
名前 / ファイル ライセンス アクション
Supplementary_Figs.pdf Supplementary_Figs.pdf ファイル公開:2018-03-01 (7.0 MB)
Figs1-7.pdf Figs1-7.pdf ファイル公開:2018-03-01 (6.8 MB)
Goto_et_al.pdf Goto_et_al.pdf ファイル公開:2018-03-01 (264.5 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-12-08
タイトル
タイトル P-class pentatricopeptide repeat protein PTSF1 is required for splicing of the plastid pre-tRNAIle in Physcomitrella patens
言語 en
著者 Goto, Seiya

× Goto, Seiya

WEKO 67877

en Goto, Seiya

Search repository
Kawaguchi, Yasuhiro

× Kawaguchi, Yasuhiro

WEKO 67878

en Kawaguchi, Yasuhiro

Search repository
Sugita, Cieko

× Sugita, Cieko

WEKO 67879

en Sugita, Cieko

Search repository
Ichinose, Mizuho

× Ichinose, Mizuho

WEKO 67880

en Ichinose, Mizuho

Search repository
Sugita, Mamoru

× Sugita, Mamoru

WEKO 67881

en Sugita, Mamoru

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
権利情報 This is the peer reviewed version of the following article: [Goto, S., Kawaguchi, Y., Sugita, C., Ichinose, M. and Sugita, M. (2016), P-class pentatricopeptide repeat protein PTSF1 is required for splicing of the plastid pre-tRNAIle in Physcomitrella patens. Plant J, 86: 493–503], which has been published in final form at [http://doi.org/10.1111/tpj.13184]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
キーワード
主題Scheme Other
主題 P-class Pentatricopeptide repeat protein
キーワード
主題Scheme Other
主題 splicing
キーワード
主題Scheme Other
主題 group-II intron
キーワード
主題Scheme Other
主題 tRNAIle-GAU
キーワード
主題Scheme Other
主題 plastid
キーワード
主題Scheme Other
主題 NEP-dependent transcription
抄録
内容記述 Pentatricopeptide repeat (PPR) proteins are widely distributed in eukaryotes and are mostly localized in mitochondria or plastids. PPR proteins play essential roles in various RNA processing steps in organelles; however, the function of the majority of PPR proteins remains unknown. To examine the function of plastid PPR proteins, PpPPR_4 gene knock-out mutants were characterized in Physcomitrella patens. The knock-out mosses displayed severe growth retardation and reduced effective quantum yield of photosystem II. Immunoblot analysis showed that knock-out of PpPPR_4 resulted in a strongly reduced level of plastid-encoded proteins, such as photosystem II reaction center protein D1, the β subunit of ATP synthase, and the stromal enzyme, Rubisco. To further investigate whether knock-out of the PpPPR_4 gene affects plastid gene expression, we analyzed steady-state transcript levels of protein- and rRNA-coding genes by quantitative RT-PCR. This analysis showed that the level of many protein-coding transcripts increased in the mutants. In contrast, splicing of a spacer tRNAIle precursor encoded by the rrn operon was specifically impaired in the mutants, whereas the accumulation of other plastid tRNAs and rRNAs was not largely affected. Thus, the defect in tRNAIle splicing leads to a considerable reduction of mature tRNAIle, which may be accountable for the reduced protein level. An RNA mobility shift assay showed that the recombinant PpPPR_4 bound preferentially to domain III of the tRNAIle group-II intron. These results provide evidence that PpPPR_4 functions in RNA splicing of the tRNAIle intron, and hence PpPPR_4 was named plastid tRNA splicing factor 1 (PTSF1).
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/tpj.13184
ISSN
収録物識別子タイプ PISSN
収録物識別子 0960-7412
書誌情報 en : PLANT JOURNAL

巻 86, 号 6, p. 493-503, 発行日 2016-06
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1111/tpj.13184
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25167
識別子タイプ HDL
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