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Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS
http://hdl.handle.net/2237/25806
354cb2a3-2b9f-4351-ac80-e400f2550a54
名前 / ファイル | ライセンス | アクション | |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-03-24 | |||||
タイトル | ||||||
タイトル | Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS | |||||
著者 |
Ito, Tomokazu
× Ito, Tomokazu× Yamauchi, Ayako× Hemmi, Hisashi× Yoshimura, Tohru |
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権利 | ||||||
権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pyridoxal 5′-phosphate | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ophthalmic acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | YggS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Escherichia coli | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glutathione | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Amino acid | |||||
抄録 | ||||||
内容記述 | Escherichia coli YggS is a highly conserved pyridoxal 5′-phosphate (PLP)-binding protein whose biochemical function is currently unknown. A previous study with a yggS-deficient E. coli strain (ΔyggS) demonstrated that YggS controls l-Ile- and l-Val-metabolism by modulating 2-ketobutyrate (2-KB), l-2-aminobutyrate (l-2-AB), and/or coenzyme A (CoA) availability in a PLP-dependent fashion. In this study, we found that ΔyggS accumulates an unknown metabolite as judged by amino acid analyses. LC/MS and MS/MS analyses of the compound with propyl chloroformate derivatization, and co-chromatography analysis identified this compound as γ-l-glutamyl-l-2-aminobutyryl-glycine (ophthalmic acid), a glutathione (GSH) analogue in which the l-Cys moiety is replaced by l-2-AB. We also determine the metabolic consequence of the yggS mutation. Absence of YggS initially increases l-2-AB availability, and then causes ophthalmic acid accumulation and CoA limitation in the cell. The expression of a γ-glutamylcysteine synthetase and a glutathione synthetase in a ΔyggS background causes high-level accumulation of ophthalmic acid in the cells (∼1.2 nmol/mg cells) in a minimal synthetic medium. This opens the possibility of a first fermentative production of ophthalmic acid. | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1389-1723 | |||||
書誌情報 |
Journal of Bioscience and Bioengineering 巻 122, 号 6, p. 689-693, 発行日 2016-12 |
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著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | http://dx.doi.org/10.1016/j.jbiosc.2016.06.010 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/25806 | |||||
識別子タイプ | HDL |