ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "d4350cb9-bb66-42e2-9f9d-8052db84c2ae"}, "_deposit": {"id": "23609", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23609"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00023609", "sets": ["667"]}, "author_link": ["70138", "70139", "70140", "70141"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "693", "bibliographicPageStart": "689", "bibliographicVolumeNumber": "122", "bibliographic_titles": [{"bibliographic_title": "Journal of Bioscience and Bioengineering", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Escherichia coli YggS is a highly conserved pyridoxal 5′-phosphate (PLP)-binding protein whose biochemical function is currently unknown. A previous study with a yggS-deficient E. coli strain (ΔyggS) demonstrated that YggS controls l-Ile- and l-Val-metabolism by modulating 2-ketobutyrate (2-KB), l-2-aminobutyrate (l-2-AB), and/or coenzyme A (CoA) availability in a PLP-dependent fashion. In this study, we found that ΔyggS accumulates an unknown metabolite as judged by amino acid analyses. LC/MS and MS/MS analyses of the compound with propyl chloroformate derivatization, and co-chromatography analysis identified this compound as γ-l-glutamyl-l-2-aminobutyryl-glycine (ophthalmic acid), a glutathione (GSH) analogue in which the l-Cys moiety is replaced by l-2-AB. We also determine the metabolic consequence of the yggS mutation. Absence of YggS initially increases l-2-AB availability, and then causes ophthalmic acid accumulation and CoA limitation in the cell. The expression of a γ-glutamylcysteine synthetase and a glutathione synthetase in a ΔyggS background causes high-level accumulation of ophthalmic acid in the cells (∼1.2 nmol/mg cells) in a minimal synthetic medium. This opens the possibility of a first fermentative production of ophthalmic acid.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://dx.doi.org/10.1016/j.jbiosc.2016.06.010"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/25806"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.jbiosc.2016.06.010", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1389-1723", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ito, Tomokazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "70138", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamauchi, Ayako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "70139", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hemmi, Hisashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "70140", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshimura, Tohru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "70141", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "yggs2_revise.pdf", "filesize": [{"value": "694.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 694000.0, "url": {"label": "yggs2_revise.pdf ファイル公開:2017/12/01", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/23609/files/yggs2_revise.pdf"}, "version_id": "e0c9162e-77ce-4adf-b4a5-23ca791b61b1"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "YggS_second-revise.pdf", "filesize": [{"value": "421.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 421300.0, "url": {"label": "YggS_second-revise.pdf ファイル公開:2017/12/01", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/23609/files/YggS_second-revise.pdf"}, "version_id": "055fdc0b-ab5d-43c4-b9c2-d4502c78457d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Pyridoxal 5′-phosphate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ophthalmic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "YggS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Escherichia coli", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glutathione", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Amino acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/25806", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-03-24"}, "publish_date": "2017-03-24", "publish_status": "0", "recid": "23609", "relation": {}, "relation_version_is_last": true, "title": ["Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS"], "weko_shared_id": -1}
  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS

http://hdl.handle.net/2237/25806
http://hdl.handle.net/2237/25806
354cb2a3-2b9f-4351-ac80-e400f2550a54
名前 / ファイル ライセンス アクション
yggs2_revise.pdf yggs2_revise.pdf ファイル公開:2017/12/01 (694.0 kB)
YggS_second-revise.pdf YggS_second-revise.pdf ファイル公開:2017/12/01 (421.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-03-24
タイトル
タイトル Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS
言語 en
著者 Ito, Tomokazu

× Ito, Tomokazu

WEKO 70138

en Ito, Tomokazu

Search repository
Yamauchi, Ayako

× Yamauchi, Ayako

WEKO 70139

en Yamauchi, Ayako

Search repository
Hemmi, Hisashi

× Hemmi, Hisashi

WEKO 70140

en Hemmi, Hisashi

Search repository
Yoshimura, Tohru

× Yoshimura, Tohru

WEKO 70141

en Yoshimura, Tohru

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Pyridoxal 5′-phosphate
キーワード
主題Scheme Other
主題 Ophthalmic acid
キーワード
主題Scheme Other
主題 YggS
キーワード
主題Scheme Other
主題 Escherichia coli
キーワード
主題Scheme Other
主題 Glutathione
キーワード
主題Scheme Other
主題 Amino acid
抄録
内容記述 Escherichia coli YggS is a highly conserved pyridoxal 5′-phosphate (PLP)-binding protein whose biochemical function is currently unknown. A previous study with a yggS-deficient E. coli strain (ΔyggS) demonstrated that YggS controls l-Ile- and l-Val-metabolism by modulating 2-ketobutyrate (2-KB), l-2-aminobutyrate (l-2-AB), and/or coenzyme A (CoA) availability in a PLP-dependent fashion. In this study, we found that ΔyggS accumulates an unknown metabolite as judged by amino acid analyses. LC/MS and MS/MS analyses of the compound with propyl chloroformate derivatization, and co-chromatography analysis identified this compound as γ-l-glutamyl-l-2-aminobutyryl-glycine (ophthalmic acid), a glutathione (GSH) analogue in which the l-Cys moiety is replaced by l-2-AB. We also determine the metabolic consequence of the yggS mutation. Absence of YggS initially increases l-2-AB availability, and then causes ophthalmic acid accumulation and CoA limitation in the cell. The expression of a γ-glutamylcysteine synthetase and a glutathione synthetase in a ΔyggS background causes high-level accumulation of ophthalmic acid in the cells (∼1.2 nmol/mg cells) in a minimal synthetic medium. This opens the possibility of a first fermentative production of ophthalmic acid.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.jbiosc.2016.06.010
ISSN
収録物識別子タイプ PISSN
収録物識別子 1389-1723
書誌情報 en : Journal of Bioscience and Bioengineering

巻 122, 号 6, p. 689-693, 発行日 2016-12
著者版フラグ
値 author
URI
識別子 http://dx.doi.org/10.1016/j.jbiosc.2016.06.010
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25806
識別子タイプ HDL
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 14:29:26.451775
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3