{"_buckets": {"deposit": "d4350cb9-bb66-42e2-9f9d-8052db84c2ae"}, "_deposit": {"id": "23609", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23609"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00023609"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "693", "bibliographicPageStart": "689", "bibliographicVolumeNumber": "122", "bibliographic_titles": [{"bibliographic_title": "Journal of Bioscience and Bioengineering"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Escherichia coli YggS is a highly conserved pyridoxal 5\u2032-phosphate (PLP)-binding protein whose biochemical function is currently unknown. A previous study with a yggS-deficient E. coli strain (\u0394yggS) demonstrated that YggS controls l-Ile- and l-Val-metabolism by modulating 2-ketobutyrate (2-KB), l-2-aminobutyrate (l-2-AB), and/or coenzyme A (CoA) availability in a PLP-dependent fashion. In this study, we found that \u0394yggS accumulates an unknown metabolite as judged by amino acid analyses. LC/MS and MS/MS analyses of the compound with propyl chloroformate derivatization, and co-chromatography analysis identified this compound as \u03b3-l-glutamyl-l-2-aminobutyryl-glycine (ophthalmic acid), a glutathione (GSH) analogue in which the l-Cys moiety is replaced by l-2-AB. We also determine the metabolic consequence of the yggS mutation. Absence of YggS initially increases l-2-AB availability, and then causes ophthalmic acid accumulation and CoA limitation in the cell. The expression of a \u03b3-glutamylcysteine synthetase and a glutathione synthetase in a \u0394yggS background causes high-level accumulation of ophthalmic acid in the cells (\u223c1.2 nmol/mg cells) in a minimal synthetic medium. This opens the possibility of a first fermentative production of ophthalmic acid.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://dx.doi.org/10.1016/j.jbiosc.2016.06.010"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/25806"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1389-1723", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ito, Tomokazu"}], "nameIdentifiers": [{"nameIdentifier": "70138", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamauchi, Ayako"}], "nameIdentifiers": [{"nameIdentifier": "70139", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hemmi, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "70140", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshimura, Tohru"}], "nameIdentifiers": [{"nameIdentifier": "70141", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "yggs2_revise.pdf", "filesize": [{"value": "694.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 694000.0, "url": {"label": "yggs2_revise.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2017/12/01", "url": "https://nagoya.repo.nii.ac.jp/record/23609/files/yggs2_revise.pdf"}, "version_id": "e0c9162e-77ce-4adf-b4a5-23ca791b61b1"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-12-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "YggS_second-revise.pdf", "filesize": [{"value": "421.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 421300.0, "url": {"label": "YggS_second-revise.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2017/12/01", "url": "https://nagoya.repo.nii.ac.jp/record/23609/files/YggS_second-revise.pdf"}, "version_id": "055fdc0b-ab5d-43c4-b9c2-d4502c78457d"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Pyridoxal 5\u2032-phosphate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ophthalmic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "YggS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Escherichia coli", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glutathione", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Amino acid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5\u2032-phosphate-binding protein YggS", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5\u2032-phosphate-binding protein YggS"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/25806", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-03-24"}, "publish_date": "2017-03-24", "publish_status": "0", "recid": "23609", "relation": {}, "relation_version_is_last": true, "title": ["Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5\u2032-phosphate-binding protein YggS"], "weko_shared_id": null}