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Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2)
http://hdl.handle.net/2237/26612
http://hdl.handle.net/2237/266129b6568fc-97d8-4116-bb7d-cf7f3beca5eb
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Hitomi_BBB_Takada_Nagoya_Univ.pdf ファイル公開：2018/01/01 (1.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-06-09 | |||||
| タイトル | ||||||
| タイトル | Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) | |||||
| 言語 | en | |||||
| 著者 |
Takada, Yuki
× Takada, Yuki× Watanabe, Yuko× Okuya, Kazuho× Tatsukawa, Hideki× Hashimoto, Hisashi× Hitomi, Kiyotaka |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | (c)日本農芸化学会. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Bioscience, Biotechnology, and Biochemistry on 18/11/2016, available online: http://www.tandfonline.com/10.1080/09168451.2016.1256757. | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Transglutaminase is an enzyme family responsible for post-translational modification such as protein cross-linking and the attachment of primary amine and/or deamidation of glutamine-residue in proteins. Medaka (Oryzias latipes), a recently established model fish, has similar functional proteins to those characterized in mammals. Previously, we found the apparent orthologues that correspond to human transglutaminases in medaka. In this study, regarding the medaka orthologue of human tissue-type transglutaminase (OlTGT), recombinant protein was expressed in an active form in bacteria cultured at low temperature. Using the recombinant protein, we biochemically characterized the enzymatic activity and also obtained a monoclonal antibody that specifically recognized OlTGT. Immunochemical analysis revealed that OlTGT was not expressed ubiquitously, unlike its mammalian orthologue, but in primarily limited tissues such as the eye, brain, spinal cord, and gas gland. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Taylor & Francis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1080/09168451.2016.1256757 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0916-8451 | |||||
| 書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 81, 号 3, p. 469-474, 発行日 2017 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/26612 | |||||
| 識別子タイプ | HDL | |||||
