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  1. D400 創薬科学研究科
  2. D400a 雑誌掲載論文
  3. 学術雑誌

Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2)

http://hdl.handle.net/2237/26612
9b6568fc-97d8-4116-bb7d-cf7f3beca5eb
名前 / ファイル ライセンス アクション
Hitomi_BBB_Takada_Nagoya_Univ.pdf Hitomi_BBB_Takada_Nagoya_Univ.pdf ファイル公開:2018/01/01 (1.5 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-06-09
タイトル
タイトル Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2)
著者 Takada, Yuki

× Takada, Yuki

WEKO 72295

Takada, Yuki

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Watanabe, Yuko

× Watanabe, Yuko

WEKO 72296

Watanabe, Yuko

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Okuya, Kazuho

× Okuya, Kazuho

WEKO 72297

Okuya, Kazuho

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Tatsukawa, Hideki

× Tatsukawa, Hideki

WEKO 72298

Tatsukawa, Hideki

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Hashimoto, Hisashi

× Hashimoto, Hisashi

WEKO 72299

Hashimoto, Hisashi

Search repository
Hitomi, Kiyotaka

× Hitomi, Kiyotaka

WEKO 72300

Hitomi, Kiyotaka

Search repository
権利
権利情報 (c)日本農芸化学会. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Bioscience, Biotechnology, and Biochemistry on 18/11/2016, available online: http://www.tandfonline.com/10.1080/09168451.2016.1256757.
抄録
内容記述 Transglutaminase is an enzyme family responsible for post-translational modification such as protein cross-linking and the attachment of primary amine and/or deamidation of glutamine-residue in proteins. Medaka (Oryzias latipes), a recently established model fish, has similar functional proteins to those characterized in mammals. Previously, we found the apparent orthologues that correspond to human transglutaminases in medaka. In this study, regarding the medaka orthologue of human tissue-type transglutaminase (OlTGT), recombinant protein was expressed in an active form in bacteria cultured at low temperature. Using the recombinant protein, we biochemically characterized the enzymatic activity and also obtained a monoclonal antibody that specifically recognized OlTGT. Immunochemical analysis revealed that OlTGT was not expressed ubiquitously, unlike its mammalian orthologue, but in primarily limited tissues such as the eye, brain, spinal cord, and gas gland.
内容記述タイプ Abstract
出版者
出版者 Taylor & Francis
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌情報 Bioscience, Biotechnology, and Biochemistry

巻 81, 号 3, p. 469-474, 発行日 2017
著者版フラグ
値 author
URI
識別子 http://hdl.handle.net/2237/26612
識別子タイプ HDL
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