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  1. C100 医学部/医学系研究科
  2. C100a 雑誌掲載論文
  3. 学術雑誌

Mechanical stress loading induces CD44 cleavage in human chondrocytic HCS-2/8 cells

http://hdl.handle.net/2237/26633
d91be18f-d3fc-4d0f-83b4-52eccab09c6d
名前 / ファイル ライセンス アクション
Kobayakawa_BBRC_manuscript_final_version.pdf Kobayakawa_BBRC_manuscript_final_version.pdf ファイル公開:2017/09/23 (400.1 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-06-09
タイトル
タイトル Mechanical stress loading induces CD44 cleavage in human chondrocytic HCS-2/8 cells
著者 Kobayakawa, Tomonori

× Kobayakawa, Tomonori

WEKO 72359

Kobayakawa, Tomonori

Search repository
Takahashi, Nobunori

× Takahashi, Nobunori

WEKO 72360

Takahashi, Nobunori

Search repository
Sobue, Yasumori

× Sobue, Yasumori

WEKO 72361

Sobue, Yasumori

Search repository
Terabe, Kenya

× Terabe, Kenya

WEKO 72362

Terabe, Kenya

Search repository
Ishiguro, Naoki

× Ishiguro, Naoki

WEKO 72363

Ishiguro, Naoki

Search repository
Kojima, Toshihisa

× Kojima, Toshihisa

WEKO 72364

Kojima, Toshihisa

Search repository
権利
権利情報 © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Mechanical loading
キーワード
主題Scheme Other
主題 CD44 cleavage
キーワード
主題Scheme Other
主題 ADAM-10
キーワード
主題Scheme Other
主題 TRPV4
キーワード
主題Scheme Other
主題 HCS-2/8
キーワード
主題Scheme Other
主題 Osteoarthritis
抄録
内容記述 Although excessive mechanical stress loading is known to induce articular cartilage degradation, the mechanism underlying this process is unclear. The interaction between hyaluronan (HA) and its primary receptor CD44 maintains the homeostasis of articular chondrocytes. CD44 cleavage and the generation of CD44-intracellular domain (ICD) can lead to the loss of extracellular matrices in chondrocytes. Here we studied the effects of cyclic tensile strain (CTS) loading, a representative mechanical stress, on CD44 cleavage. CTS loading (1 Hz and 20% elongation for 48 h) increased ADAM10 expression and CD44 cleavage in HCS-2/8 cells, a human chondrocytic cell line. Co-treatment with a chemical ADAM10 inhibitor significantly suppressed CTS loading-induced CD44 cleavage. Chemical inhibition of transient receptor potential vanilloid 4 (TRPV4) significantly suppressed CTS loading-induced ADAM10 expression and CD44 cleavage. Conversely, chemical activation of TRPV4 increased ADAM10 expression and enhanced CD44 cleavage. Our findings suggest that CTS loading significantly increases the expression of ADAM10, which in turn enhances CD44 cleavage in HCS-2/8 cells. The primary mechanoreceptor mediating this process is TRPV4. This signature event could provide an avenue for intervention in the prevention of cartilage degradation leading to OA.
内容記述タイプ Abstract
出版者
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISSN
収録物識別子タイプ ISSN
収録物識別子 0006-291X
書誌情報 Biochemical and Biophysical Research Communications

巻 478, 号 3, p. 1230-1235, 発行日 2016-09-23
著者版フラグ
値 author
URI
識別子 https://doi.org/10.1016/j.bbrc.2016.08.099
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/26633
識別子タイプ HDL
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