{"_buckets": {"deposit": "9d699af9-d028-4406-8e34-a74c7f5f2dee"}, "_deposit": {"id": "24747", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24747"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024747", "sets": ["501"]}, "author_link": ["73506", "73507", "73508", "73509", "73510", "73511", "73512", "73513", "73514", "73515", "73516"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-07", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "917", "bibliographicPageStart": "904", "bibliographicVolumeNumber": "108", "bibliographic_titles": [{"bibliographic_title": "Free Radical Biology and Medicine", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Non-thermal plasma (NTP) is a potential new therapeutic modality for cancer. However, its mechanism of action remains unclear. Herein, we studied the effect of NTP on mesothelioma cells and fibroblasts to understand its anti-proliferative efficacy. Interestingly, NTP demonstrated greater selective anti-proliferative activity against mesothelioma cells relative to fibroblasts than cisplatin, which is used for mesothelioma treatment. The anti-proliferative effect of NTP was enhanced by pre-incubation with the cellular iron donor, ferric ammonium citrate (FAC), and inhibited by iron chelation using desferrioxamine (DFO). Three oxidative stress probes (CM-H2DCFDA, MitoSOX and C11-BODIPY) demonstrated reactive oxygen species (ROS) generation by NTP, which was inhibited by DFO. Moreover, NTP decreased transferrin receptor-1 and increased ferritin-H and -L chain expression that was correlated with decreased iron-regulatory protein expression and RNA-binding activity. This regulation was potentially due to increased intracellular iron in lysosomes, which was demonstrated via the Fe(II)-selective probe, HMRhoNox-M, and was consistent with autophagic-induction. Immunofluorescence using LysoTracker and Pepstatin A probes demonstrated increased cellular lysosome content, which was confirmed by elevated LAMP1 expression. The enhanced lysosomal biogenesis after NTP could be due to the observed increase in fluid-phase endocytosis and early endosome formation. These results suggest NTP acts as a stressor, which results in increased endocytosis, lysosome content and autophagy. In fact, NTP rapidly increased autophagosome formation, as judged by increased LC3B-II expression, which co-localized with LAMP1, indicating autophagolysosome formation. Autophagic-induction by NTP was confirmed using electron microscopy. In summary, NTP acts as a cellular stressor to rapidly induce fluid-phase endocytosis, lysosome biogenesis and autophagy.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "https://doi.org/10.1016/j.freeradbiomed.2017.04.368"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/26969"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.freeradbiomed.2017.04.368", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0891-5849", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shi, Lei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73506", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Fumiya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73507", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Wang, Yue", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73508", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okazaki, Yasumasa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73509", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Hiromasa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73510", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mizuno, Masaaki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73511", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hori, Masaru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73512", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirayama, Tasuku", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73513", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagasawa, Hideko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73514", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Richardson, Des R.", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73515", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Toyokuni, Shinya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "73516", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-07-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Lei_Shi_FRBM_Plasma_2017_Repository.pdf", "filesize": [{"value": "5.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 5000000.0, "url": {"label": "Lei_Shi_FRBM_Plasma_2017_Repository.pdf ファイル公開：2018/07/01", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/24747/files/Lei_Shi_FRBM_Plasma_2017_Repository.pdf"}, "version_id": "7f4c8553-65f3-47ff-9f18-c4f2d94ab905"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["501"], "permalink_uri": "http://hdl.handle.net/2237/26969", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-09-06"}, "publish_date": "2017-09-06", "publish_status": "0", "recid": "24747", "relation": {}, "relation_version_is_last": true, "title": ["Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy"], "weko_shared_id": -1}