{"_buckets": {"deposit": "c0d6d0fb-4f32-4f6f-aa0f-1c6af7a33c48"}, "_deposit": {"id": "24897", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24897"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024897"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "486", "bibliographicPageStart": "477", "bibliographicVolumeNumber": "79", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Indoxyl sulfate (IS) induces fibrosis and inflammation in kidneys via oxidative stress through the induction of transforming growth factor-\u03b21 (TGF-\u03b21) and monocyte chemotactic protein-1 (MCP-1). Furthermore, IS is a potent endogenous agonist for aryl hydrocarbon receptor (AHR), which regulates the transcription of genes such as cytochrome P450 (CYP) 1A1. Indole-3-propionic acid (IPA) is an antioxidant and has been reported to be neuroprotective. We determined whether IPA suppresses IS-induced expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 in proximal tubular cells. The effects of IS on the expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 were studied using normotensive rats and hypertensive rats. The effects of IPA on IS-induced expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 were studied using proximal tubular cells (HK-2). Furthermore, the effects of IPA on IS-induced expression and phosphorylation of signal transducer and activator of transcription 3 (Stat3) were studied in HK-2 cells. Administration of IS induced the expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 in the tubular cells of rat kidneys. IPA significantly suppressed IS-induced mRNA and protein expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 in HK-2 cells. IPA suppressed the IS-induced expression and phosphorylation of Stat3 in HK-2 cells. Furthermore, knockdown of Stat3 inhibited the IS-induced mRNA and protein expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 in HK-2 cells. In conclusion, IPA suppressed the IS-induced expression of AHR, CYP1A1, TGF-\u03b21, and MCP-1 through suppression of Stat3 in proximal tubular cells. Thus, IPA suppresses IS-induced expression of fibrotic and inflammatory genes in proximal tubular cells.", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "This study was supported in part by JSPS research grant (JSPS KAKENHI Grant Number 15J00397).", "subitem_description_type": "Other"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "URI", "subitem_identifier_uri": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/794.html"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27117"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.79.4.477", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University Graduate School of Medicine, School of Medicine"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Yisireyili, Maimaiti"}], "nameIdentifiers": [{"nameIdentifier": "74127", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeshita, Kyosuke"}], "nameIdentifiers": [{"nameIdentifier": "74128", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito, Shinichi"}], "nameIdentifiers": [{"nameIdentifier": "74129", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murohara, Toyoaki"}], "nameIdentifiers": [{"nameIdentifier": "74130", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Niwa, Toshimitsu"}], "nameIdentifiers": [{"nameIdentifier": "74131", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "07_Yisireyli.pdf", "filesize": [{"value": "11.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 11500000.0, "url": {"label": "07_Yisireyli.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/24897/files/07_Yisireyli.pdf"}, "version_id": "f05d0f1d-4ae2-491d-b73e-c5293752a789"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "indole-3-propionic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "indoxyl sulfate", "subitem_subject_scheme": "Other"}, {"subitem_subject": "aryl hydrocarbon receptor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "TGF-\u03b21", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proximal tubular cells", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Indole-3-propionic acid suppresses indoxyl sulfate-induced expression of fibrotic and inflammatory genes in proximal tubular cells", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Indole-3-propionic acid suppresses indoxyl sulfate-induced expression of fibrotic and inflammatory genes in proximal tubular cells"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/2043"], "permalink_uri": "https://doi.org/10.18999/nagjms.79.4.477", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-11-22"}, "publish_date": "2017-11-22", "publish_status": "0", "recid": "24897", "relation": {}, "relation_version_is_last": true, "title": ["Indole-3-propionic acid suppresses indoxyl sulfate-induced expression of fibrotic and inflammatory genes in proximal tubular cells"], "weko_shared_id": 3}