{"_buckets": {"deposit": "bcf7aeab-4e4e-4a89-a15f-f0153f6de665"}, "_deposit": {"id": "24950", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24950"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024950", "sets": ["696"]}, "author_link": ["74381", "74382"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-09-23", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "613", "bibliographicPageStart": "609", "bibliographicVolumeNumber": "491", "bibliographic_titles": [{"bibliographic_title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Ran-binding protein 3 (RanBP3) is a primarily nuclear Ran-binding protein that functions as an accessory factor in the Ran GTPase system. RanBP3 associates with Ran-specific nucleotide exchange factor RCC1 and enhances its catalytic activity towards Ran. RanBP3 also promotes CRM1-mediated nuclear export as well as CRM1-independent nuclear export of β-catenin, Smad2, and Smad3. Nuclear import of RanBP3 is dependent on the nuclear import adaptor protein importin-α and, RanBP3 is imported more efficiently by importin-α3 than by other members of the importin-α family. Protein kinase signaling pathways control nucleocytoplasmic transport through phosphorylation of RanBP3 at Ser58, immediately C-terminal to the nuclear localization signal (NLS) in the N-terminal region of RanBP3. Here we report the crystal structure of human importin-α3 bound to an N-terminal fragment of human RanBP3 containing the NLS sequence that is necessary and sufficient for nuclear import. The structure reveals that RanBP3 binds to importin-α3 residues that are strictly conserved in all seven isoforms of human importin-α at the major NLS-binding site, indicating that the region of importin-α outside the NLS-binding site, possibly the autoinhibotory importin-β1-binding domain, may be the key determinant for the preferential binding of RanBP3 to importin-α3. Computational docking simulation indicates that phosphorylation of RanBP3 at Ser58 could potentially stabilize the association of RanBP3 with importin-α through interactions between the phosphate moiety of phospho-Ser58 of RanBP3 and a cluster of basic residues (Arg96 and Lys97 in importin-α3) on armadillo repeat 1 of importin-α.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1016/j.bbrc.2017.07.155"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27172"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbrc.2017.07.155", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Koyama, Masako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74381", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuura, Yoshiyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74382", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-09-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "koyama2017BBRC_for_depository.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "koyama2017BBRC_for_depository.pdf ファイル公開日:2018/09/23", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/24950/files/koyama2017BBRC_for_depository.pdf"}, "version_id": "7d154348-b328-44dc-a434-8e357429bb79"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "nuclear import", "subitem_subject_scheme": "Other"}, {"subitem_subject": "importin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NLS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RanBP3", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phosphorylation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["696"], "permalink_uri": "http://hdl.handle.net/2237/27172", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-12-19"}, "publish_date": "2017-12-19", "publish_status": "0", "recid": "24950", "relation": {}, "relation_version_is_last": true, "title": ["Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3"], "weko_shared_id": -1}