{"_buckets": {"deposit": "bcf7aeab-4e4e-4a89-a15f-f0153f6de665"}, "_deposit": {"id": "24950", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24950"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024950"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-09-23", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "613", "bibliographicPageStart": "609", "bibliographicVolumeNumber": "491", "bibliographic_titles": [{"bibliographic_title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Ran-binding protein 3 (RanBP3) is a primarily nuclear Ran-binding protein that functions as an accessory factor in the Ran GTPase system. RanBP3 associates with Ran-specific nucleotide exchange factor RCC1 and enhances its catalytic activity towards Ran. RanBP3 also promotes CRM1-mediated nuclear export as well as CRM1-independent nuclear export of \u03b2-catenin, Smad2, and Smad3. Nuclear import of RanBP3 is dependent on the nuclear import adaptor protein importin-\u03b1 and, RanBP3 is imported more efficiently by importin-\u03b13 than by other members of the importin-\u03b1 family. Protein kinase signaling pathways control nucleocytoplasmic transport through phosphorylation of RanBP3 at Ser58, immediately C-terminal to the nuclear localization signal (NLS) in the N-terminal region of RanBP3. Here we report the crystal structure of human importin-\u03b13 bound to an N-terminal fragment of human RanBP3 containing the NLS sequence that is necessary and sufficient for nuclear import. The structure reveals that RanBP3 binds to importin-\u03b13 residues that are strictly conserved in all seven isoforms of human importin-\u03b1 at the major NLS-binding site, indicating that the region of importin-\u03b1 outside the NLS-binding site, possibly the autoinhibotory importin-\u03b21-binding domain, may be the key determinant for the preferential binding of RanBP3 to importin-\u03b13. Computational docking simulation indicates that phosphorylation of RanBP3 at Ser58 could potentially stabilize the association of RanBP3 with importin-\u03b1 through interactions between the phosphate moiety of phospho-Ser58 of RanBP3 and a cluster of basic residues (Arg96 and Lys97 in importin-\u03b13) on armadillo repeat 1 of importin-\u03b1.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1016/j.bbrc.2017.07.155"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27172"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Koyama, Masako"}], "nameIdentifiers": [{"nameIdentifier": "74381", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuura, Yoshiyuki"}], "nameIdentifiers": [{"nameIdentifier": "74382", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-09-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "koyama2017BBRC_for_depository.pdf", "filesize": [{"value": "2.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2000000.0, "url": {"label": "koyama2017BBRC_for_depository.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2018/09/23", "url": "https://nagoya.repo.nii.ac.jp/record/24950/files/koyama2017BBRC_for_depository.pdf"}, "version_id": "7d154348-b328-44dc-a434-8e357429bb79"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "nuclear import", "subitem_subject_scheme": "Other"}, {"subitem_subject": "importin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NLS", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RanBP3", "subitem_subject_scheme": "Other"}, {"subitem_subject": "phosphorylation", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3"}]}, "item_type_id": "10", "owner": "1", "path": ["336/695/696"], "permalink_uri": "http://hdl.handle.net/2237/27172", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2017-12-19"}, "publish_date": "2017-12-19", "publish_status": "0", "recid": "24950", "relation": {}, "relation_version_is_last": true, "title": ["Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3"], "weko_shared_id": null}