{"_buckets": {"deposit": "d2e2cff4-16d5-479d-9395-58e8d736a0be"}, "_deposit": {"id": "25215", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25215"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025215"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "899", "bibliographicPageStart": "885", "bibliographicVolumeNumber": "22", "bibliographic_titles": [{}, {"bibliographic_title": "Genes to Cells", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Milk fat globule-EGF factor 8 (MFG-E8) is a divalent-binding secretory protein possessing an Arg-Gly-Asp (RGD) motif and a phosphatidylserine (PS)-binding motif. This protein has been shown to be involved in mammary gland development and morphogenesis. Integrin-binding activity is necessary for these MFG-E8-dependent cell processes. Although the target cells and molecules of MFG-E8 in the cellular microenvironment are important to understand its physiological function, its localization is largely unclear. Here, we found that mouse MFG-E8 localized to the basal lamina of the mammary gland during involution. In a model system of mammary COMMA-1D cells, exogenously and endogenously expressed MFG-E8 was deposited in the extracellular matrix (ECM) with membranous particles dependently on the PS-binding motifs in the discoidin domains that were essential for association ability to extracellular vesicles (EVs). These data showed the basal MFG-E8 localization mechanism in which EVs served as a scaffold. Such an immobilized MFG-E8 associating with cell substrata but not soluble one in the culture media promoted integrin-dependent suppression of \u03b2-casein expression. These results suggest that MFG-E8 requires EVs to transduce cellular signals from the basolateral side of the adhesion cells by accumulating in ECM.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5: 2018/10/01", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1111/gtc.12521", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "This is the pre-peer reviewed version of the following article: [Ooishi, T., Nadano, D., Matsuda, T. and Oshima, K. (2017), Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells. Genes Cells, 22: 885\u2013899. doi:10.1111/gtc.12521], which has been published in final form at [http://doi.org/10.1111/gtc.12521]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1356-9597", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ooishi, Takuya"}], "nameIdentifiers": [{"nameIdentifier": "75272", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nadano, Daita"}], "nameIdentifiers": [{"nameIdentifier": "75273", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuda, Tsukasa"}], "nameIdentifiers": [{"nameIdentifier": "75274", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oshima, Kenzi"}], "nameIdentifiers": [{"nameIdentifier": "75275", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-10-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Ooishi_et_al_revision_deposit_1.pdf", "filesize": [{"value": "282.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 282000.0, "url": {"label": "Ooishi_et_al_revision_deposit_1", "url": "https://nagoya.repo.nii.ac.jp/record/25215/files/Ooishi_et_al_revision_deposit_1.pdf"}, "version_id": "d7d1b85d-bf35-4640-83fe-5632407cc085"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-10-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "Figs_deposit.pdf", "filesize": [{"value": "6.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 6600000.0, "url": {"label": "Figs_deposit", "url": "https://nagoya.repo.nii.ac.jp/record/25215/files/Figs_deposit.pdf"}, "version_id": "78aec5e5-e4e8-40d3-9b9c-dd4c74824491"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "MFG-E8", "subitem_subject_scheme": "Other"}, {"subitem_subject": "extracellular matrix", "subitem_subject_scheme": "Other"}, {"subitem_subject": "extracellular vesicle", "subitem_subject_scheme": "Other"}, {"subitem_subject": "integrin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "mammary gland", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/00027430", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2018-03-15"}, "publish_date": "2018-03-15", "publish_status": "0", "recid": "25215", "relation": {}, "relation_version_is_last": true, "title": ["Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells"], "weko_shared_id": null}