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Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells
http://hdl.handle.net/2237/00027430
86b63a98-1f63-4dfe-8052-91b7ac25c42f
名前 / ファイル | ライセンス | アクション | |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-03-15 | |||||
タイトル | ||||||
タイトル | Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells | |||||
著者 |
Ooishi, Takuya
× Ooishi, Takuya× Nadano, Daita× Matsuda, Tsukasa× Oshima, Kenzi |
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権利 | ||||||
権利情報 | This is the pre-peer reviewed version of the following article: [Ooishi, T., Nadano, D., Matsuda, T. and Oshima, K. (2017), Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells. Genes Cells, 22: 885–899. doi:10.1111/gtc.12521], which has been published in final form at [http://doi.org/10.1111/gtc.12521]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | MFG-E8 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | extracellular matrix | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | extracellular vesicle | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | integrin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mammary gland | |||||
抄録 | ||||||
内容記述 | Milk fat globule-EGF factor 8 (MFG-E8) is a divalent-binding secretory protein possessing an Arg-Gly-Asp (RGD) motif and a phosphatidylserine (PS)-binding motif. This protein has been shown to be involved in mammary gland development and morphogenesis. Integrin-binding activity is necessary for these MFG-E8-dependent cell processes. Although the target cells and molecules of MFG-E8 in the cellular microenvironment are important to understand its physiological function, its localization is largely unclear. Here, we found that mouse MFG-E8 localized to the basal lamina of the mammary gland during involution. In a model system of mammary COMMA-1D cells, exogenously and endogenously expressed MFG-E8 was deposited in the extracellular matrix (ECM) with membranous particles dependently on the PS-binding motifs in the discoidin domains that were essential for association ability to extracellular vesicles (EVs). These data showed the basal MFG-E8 localization mechanism in which EVs served as a scaffold. Such an immobilized MFG-E8 associating with cell substrata but not soluble one in the culture media promoted integrin-dependent suppression of β-casein expression. These results suggest that MFG-E8 requires EVs to transduce cellular signals from the basolateral side of the adhesion cells by accumulating in ECM. | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | ファイル公開日: 2018/10/01 | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
出版者 | Wiley | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
DOI | ||||||
関連識別子 | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1111/gtc.12521 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1356-9597 | |||||
書誌情報 |
en : Genes to Cells 巻 22, 号 10, p. 885-899, 発行日 2017-10 |
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著者版フラグ | ||||||
値 | author |