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Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells
http://hdl.handle.net/2237/00027430
http://hdl.handle.net/2237/0002743086b63a98-1f63-4dfe-8052-91b7ac25c42f
| 名前 / ファイル | ライセンス | アクション |
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Ooishi_et_al_revision_deposit_1 (282.0 kB)
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Figs_deposit (6.6 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-03-15 | |||||
| タイトル | ||||||
| タイトル | Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells | |||||
| 言語 | en | |||||
| 著者 |
Ooishi, Takuya
× Ooishi, Takuya× Nadano, Daita× Matsuda, Tsukasa× Oshima, Kenzi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 権利情報 | This is the pre-peer reviewed version of the following article: [Ooishi, T., Nadano, D., Matsuda, T. and Oshima, K. (2017), Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells. Genes Cells, 22: 885–899. doi:10.1111/gtc.12521], which has been published in final form at [http://doi.org/10.1111/gtc.12521]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
| 言語 | en | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | MFG-E8 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | extracellular matrix | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | extracellular vesicle | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | integrin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mammary gland | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Milk fat globule-EGF factor 8 (MFG-E8) is a divalent-binding secretory protein possessing an Arg-Gly-Asp (RGD) motif and a phosphatidylserine (PS)-binding motif. This protein has been shown to be involved in mammary gland development and morphogenesis. Integrin-binding activity is necessary for these MFG-E8-dependent cell processes. Although the target cells and molecules of MFG-E8 in the cellular microenvironment are important to understand its physiological function, its localization is largely unclear. Here, we found that mouse MFG-E8 localized to the basal lamina of the mammary gland during involution. In a model system of mammary COMMA-1D cells, exogenously and endogenously expressed MFG-E8 was deposited in the extracellular matrix (ECM) with membranous particles dependently on the PS-binding motifs in the discoidin domains that were essential for association ability to extracellular vesicles (EVs). These data showed the basal MFG-E8 localization mechanism in which EVs served as a scaffold. Such an immobilized MFG-E8 associating with cell substrata but not soluble one in the culture media promoted integrin-dependent suppression of β-casein expression. These results suggest that MFG-E8 requires EVs to transduce cellular signals from the basolateral side of the adhesion cells by accumulating in ECM. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ファイル公開日: 2018/10/01 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Wiley | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/gtc.12521 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1356-9597 | |||||
| 書誌情報 |
en : Genes to Cells 巻 22, 号 10, p. 885-899, 発行日 2017-10 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
