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Optimized expression and purification of NavAb provide the structural insight into the voltage dependence
http://hdl.handle.net/2237/00027957
http://hdl.handle.net/2237/000279579b9933eb-ad00-4547-9c4e-eff4fba842cc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Revised_FEBSL-17-1362_R1_proof_kirie_docx (3.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-05-10 | |||||
| タイトル | ||||||
| タイトル | Optimized expression and purification of NavAb provide the structural insight into the voltage dependence | |||||
| 言語 | en | |||||
| 著者 |
Irie, Katsumasa
× Irie, Katsumasa× Haga, Yukari× Shimomura, Takushi× Fujiyoshi, Yoshinori |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | This is the peer reviewed version of the following article: [Irie, K. , Haga, Y. , Shimomura, T. and Fujiyoshi, Y. (2018), Optimized expression and purification of NavAb provide the structural insight into the voltage dependence. FEBS Lett, 592: 274-283. doi:10.1002/1873-3468.12955], which has been published in final form at [https://doi.org/10.1002/1873-3468.12955]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | protein expression | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | structural physiology | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | voltage‐gated sodium channel | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Voltage‐gated sodium channels are crucial for electro‐signalling in living systems. Analysis of the molecular mechanism requires both fine electrophysiological evaluation and high‐resolution channel structures. Here, we optimized a dual expression system of NavAb, which is a well‐established standard of prokaryotic voltage‐gated sodium channels, for E. coli and insect cells using a single plasmid vector to analyse high‐resolution protein structures and measure large ionic currents. Using this expression system, we evaluated the voltage dependence and determined the crystal structures of NavAb wild‐type and two mutants, E32Q and N49K, whose voltage dependence were positively shifted and essential interactions were lost in voltage sensor domain. The structural and functional comparison elucidated the molecular mechanisms of the voltage dependence of prokaryotic voltage‐gated sodium channels. | |||||
| 言語 | en | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | ファイル公開：2019-01-09 | |||||
| 言語 | ja | |||||
| 出版者 | ||||||
| 出版者 | Wiley | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1002/1873-3468.12955 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 00145793 | |||||
| 書誌情報 |
en : FEBS Letters 巻 592, 号 2, p. 274-283, 発行日 2018-01 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
