{"_buckets": {"deposit": "858c2552-ffa0-4cf8-a24b-04f57c524b08"}, "_deposit": {"id": "25752", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25752"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025752"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "283", "bibliographicPageStart": "274", "bibliographicVolumeNumber": "592", "bibliographic_titles": [{"bibliographic_title": "FEBS Letters"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Voltage\u2010gated sodium channels are crucial for electro\u2010signalling in living systems. Analysis of the molecular mechanism requires both fine electrophysiological evaluation and high\u2010resolution channel structures. Here, we optimized a dual expression system of NavAb, which is a well\u2010established standard of prokaryotic voltage\u2010gated sodium channels, for E. coli and insect cells using a single plasmid vector to analyse high\u2010resolution protein structures and measure large ionic currents. Using this expression system, we evaluated the voltage dependence and determined the crystal structures of NavAb wild\u2010type and two mutants, E32Q and N49K, whose voltage dependence were positively shifted and essential interactions were lost in voltage sensor domain. The structural and functional comparison elucidated the molecular mechanisms of the voltage dependence of prokaryotic voltage\u2010gated sodium channels.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2019-01-09 ", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/1873-3468.12955", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\"This is the peer reviewed version of the following article: [Irie, K. , Haga, Y. , Shimomura, T. and Fujiyoshi, Y. (2018), Optimized expression and purification of NavAb provide the structural insight into the voltage dependence. FEBS Lett, 592: 274-283. doi:10.1002/1873-3468.12955], which has been published in final form at [https://doi.org/10.1002/1873-3468.12955]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.\""}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00145793", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Irie, Katsumasa"}], "nameIdentifiers": [{"nameIdentifier": "76401", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Haga, Yukari"}], "nameIdentifiers": [{"nameIdentifier": "76402", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shimomura, Takushi"}], "nameIdentifiers": [{"nameIdentifier": "76403", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujiyoshi, Yoshinori"}], "nameIdentifiers": [{"nameIdentifier": "76404", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-01-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Revised_FEBSL-17-1362_R1_proof_kirie_docx.pdf", "filesize": [{"value": "3.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3000000.0, "url": {"label": "Revised_FEBSL-17-1362_R1_proof_kirie_docx", "url": "https://nagoya.repo.nii.ac.jp/record/25752/files/Revised_FEBSL-17-1362_R1_proof_kirie_docx.pdf"}, "version_id": "e1b8576d-2017-4c85-97f1-b1a3c982ab58"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "protein expression", "subitem_subject_scheme": "Other"}, {"subitem_subject": "structural physiology", "subitem_subject_scheme": "Other"}, {"subitem_subject": "voltage\u2010gated sodium channel", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Optimized expression and purification of NavAb provide the structural insight into the voltage dependence", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Optimized expression and purification of NavAb provide the structural insight into the voltage dependence"}]}, "item_type_id": "10", "owner": "1", "path": ["2081/2082/2083"], "permalink_uri": "http://hdl.handle.net/2237/00027957", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2018-05-10"}, "publish_date": "2018-05-10", "publish_status": "0", "recid": "25752", "relation": {}, "relation_version_is_last": true, "title": ["Optimized expression and purification of NavAb provide the structural insight into the voltage dependence"], "weko_shared_id": null}