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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum

http://hdl.handle.net/2237/00028582
http://hdl.handle.net/2237/00028582
edb271b4-f188-4c76-8321-bec0d64519bf
名前 / ファイル ライセンス アクション
Identification_and_characterization_of_small_molecule_compounds.pdf Identification_and_characterization_of_small_molecule_compounds (3.2 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-09-27
タイトル
タイトル Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum
言語 en
著者 Maeda, Kazuyuki

× Maeda, Kazuyuki

WEKO 85359

en Maeda, Kazuyuki

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Ichikawa, Hinayo

× Ichikawa, Hinayo

WEKO 85360

en Ichikawa, Hinayo

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Nakajima, Yuichi

× Nakajima, Yuichi

WEKO 85361

en Nakajima, Yuichi

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Motoyama, Takayuki

× Motoyama, Takayuki

WEKO 85362

en Motoyama, Takayuki

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Ohsato, Shuichi

× Ohsato, Shuichi

WEKO 85363

en Ohsato, Shuichi

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Kanamaru, Kyoko

× Kanamaru, Kyoko

WEKO 85364

en Kanamaru, Kyoko

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Kobayashi, Tetsuo

× Kobayashi, Tetsuo

WEKO 85365

en Kobayashi, Tetsuo

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Nishiuchi, Takumi

× Nishiuchi, Takumi

WEKO 85366

en Nishiuchi, Takumi

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Osada, Hiroyuki

× Osada, Hiroyuki

WEKO 85367

en Osada, Hiroyuki

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Kimura, Makoto

× Kimura, Makoto

WEKO 85368

en Kimura, Makoto

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright© American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/articlesonrequest/AOR-eg9SCN4f38Gp55nxRPq7
抄録
内容記述 From the RIKEN Natural Products Depository (NPDepo) chemical library, we identified small molecules that alter trichothecene 15-acetyldeoxynivalenol (15-ADON) production by Fusarium graminearum. Among trichothecene production activators, a furanocoumarin NPD12671 showed the strongest stimulatory activity on 15-ADON production by the fungus cultured in a 24-well plate. NPD12671 significantly increased the transcription of Tri6, a transcription factor gene necessary for trichothecene biosynthesis, in both trichothecene-inducing and noninducing culture conditions. Dihydroartemisinin (DHA) was identified as the most effective inhibitor of trichothecene production in 24-well plate culture; DHA inhibited trichothecene production (>50% inhibition at 1 μM) without affecting fungal mass by suppressing Tri6 expression. To determine the effect of DHA on trichothecene pathway Tri gene expression, we generated a constitutively Tri6-overexpressing strain that produced 15-ADON in YG_60 medium in Erlenmeyer flasks, conditions under which no trichothecenes are produced by the wild-type. While 5 μM DHA failed to inhibit trichothecene biosynthesis by the overexpressor in trichothecene-inducing YS_60 culture, trichothecene production was suppressed in the YG_60 culture. Regardless of a high Tri6 transcript level in the constitutive overexpressor, the YG_60 culture showed reduced accumulation of Tri5 and Tri4 mRNA upon treatment with 5 μM DHA. Deletion mutants of FgOs2 were also generated and examined; both NPD12671 and DHA modulated trichothecene production as they did in the wild-type strain. These results are discussed in light of the mode of actions of these chemicals on trichothecene biosynthesis.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2019-05-18
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 ACS Publications
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1021/acschembio.8b00044
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 1554-8929
ISSN(Online)
収録物識別子タイプ EISSN
収録物識別子 1554-8937
書誌情報 en : ACS Chemical Biology

巻 13, 号 5, p. 1260-1269, 発行日 2018-05-18
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