{"_buckets": {"deposit": "0e8fa7a9-96a2-4096-958b-46ee0311f3c2"}, "_deposit": {"id": "26379", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "26379"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00026379", "sets": ["667"]}, "author_link": ["85359", "85360", "85361", "85362", "85363", "85364", "85365", "85366", "85367", "85368"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-05-18", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "1269", "bibliographicPageStart": "1260", "bibliographicVolumeNumber": "13", "bibliographic_titles": [{"bibliographic_title": "ACS Chemical Biology", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "From the RIKEN Natural Products Depository (NPDepo) chemical library, we identified small molecules that alter trichothecene 15-acetyldeoxynivalenol (15-ADON) production by Fusarium graminearum. Among trichothecene production activators, a furanocoumarin NPD12671 showed the strongest stimulatory activity on 15-ADON production by the fungus cultured in a 24-well plate. NPD12671 significantly increased the transcription of Tri6, a transcription factor gene necessary for trichothecene biosynthesis, in both trichothecene-inducing and noninducing culture conditions. Dihydroartemisinin (DHA) was identified as the most effective inhibitor of trichothecene production in 24-well plate culture; DHA inhibited trichothecene production (\u003e50% inhibition at 1 μM) without affecting fungal mass by suppressing Tri6 expression. To determine the effect of DHA on trichothecene pathway Tri gene expression, we generated a constitutively Tri6-overexpressing strain that produced 15-ADON in YG_60 medium in Erlenmeyer flasks, conditions under which no trichothecenes are produced by the wild-type. While 5 μM DHA failed to inhibit trichothecene biosynthesis by the overexpressor in trichothecene-inducing YS_60 culture, trichothecene production was suppressed in the YG_60 culture. Regardless of a high Tri6 transcript level in the constitutive overexpressor, the YG_60 culture showed reduced accumulation of Tri5 and Tri4 mRNA upon treatment with 5 μM DHA. Deletion mutants of FgOs2 were also generated and examined; both NPD12671 and DHA modulated trichothecene production as they did in the wild-type strain. These results are discussed in light of the mode of actions of these chemicals on trichothecene biosynthesis.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "ファイル公開：2019-05-18 ", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ACS Publications", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1021/acschembio.8b00044", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright© American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/articlesonrequest/AOR-eg9SCN4f38Gp55nxRPq7", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN（print）", "attribute_value_mlt": [{"subitem_source_identifier": "1554-8929", "subitem_source_identifier_type": "PISSN"}]}, "item_10_source_id_62": {"attribute_name": "ISSN（Online）", "attribute_value_mlt": [{"subitem_source_identifier": "1554-8937", "subitem_source_identifier_type": "EISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Maeda, Kazuyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85359", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ichikawa, Hinayo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85360", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakajima, Yuichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Motoyama, Takayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85362", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohsato, Shuichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85363", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kanamaru, Kyoko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85364", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Tetsuo", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85365", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishiuchi, Takumi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85366", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Osada, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85367", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kimura, Makoto", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "85368", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-05-18"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Identification_and_characterization_of_small_molecule_compounds.pdf", "filesize": [{"value": "3.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 3200000.0, "url": {"label": "Identification_and_characterization_of_small_molecule_compounds", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/26379/files/Identification_and_characterization_of_small_molecule_compounds.pdf"}, "version_id": "5c4fc8f1-f5e9-413c-a112-3d130bbd5c7b"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/00028582", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-09-27"}, "publish_date": "2018-09-27", "publish_status": "0", "recid": "26379", "relation": {}, "relation_version_is_last": true, "title": ["Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum"], "weko_shared_id": -1}