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Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis
http://hdl.handle.net/2237/00032178
http://hdl.handle.net/2237/0003217890d3b746-fae1-4239-b2a5-677d581f489f
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Identification_of_amino_acids (3.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2020-05-19 | |||||
| タイトル | ||||||
| タイトル | Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis | |||||
| 言語 | en | |||||
| 著者 |
Shiobara, Takuya
× Shiobara, Takuya× Nakajima, Yuichi× Maeda, Kazuyuki× Akasaka, Manami× Kitou, Yoshiyuki× Kanamaru, Kyoko× Ohsato, Shuichi× Kobayashi, Tetsuo× Nishiuchi, Takumi× Kimura, Makoto |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | “This is a post-peer-review, pre-copyedit version of an article published in [Antonie van Leeuwenhoek]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10482-018-1172-z”. | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | AreA transcription factor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Defined medium | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fed-batch culture | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mycotoxin biosynthesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Secondary metabolite | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Threonine metabolism | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nitrogen sources in media have a significant impact on the onset of secondary metabolism in fungi. For transcriptional activation of many nitrogen catabolic genes, an AreA transcription factor is indispensable. This also holds true for Fusarium graminearum that produces trichothecenes, an important group of mycotoxin, in axenic culture. Despite the presence of numerous consensus AreA-binding sites in the promoters of Tri genes in the trichothecene cluster core region, the effect of medium amino acids on trichothecene biosynthesis is poorly understood. In this study, we examined the effect of certain amino acids, which were predicted to activate AreA function and increase Tri gene transcription, on trichothecene production in liquid culture. By frequent monitoring and adjustments in the pH of the culture medium, including replacement of the spent medium with fresh medium, we demonstrate the suppressive effects of the amino acids, used as the sole nitrogen source, on trichothecene biosynthesis. When the medium pH was maintained at 4.0, Gly, l-Ser, and l-Thr suppressed trichothecene production by F. graminearum. Enhanced trichothecene-inducing effects were observed when the medium pH was 3.5, with only l-Thr suppressing trichothecene synthesis. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Springer | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s10482-018-1172-z | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0003-6072 | |||||
| 書誌情報 |
en : Antonie van Leeuwenhoek 巻 112, 号 3, p. 471-478, 発行日 2019-03-15 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
