{"_buckets": {"deposit": "d7320536-d586-441d-9133-e144c9fc6f50"}, "_deposit": {"id": "30061", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "30061"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00030061", "sets": ["667"]}, "author_link": ["99129", "99130", "99131", "99132"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019-12-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "296", "bibliographicPageStart": "291", "bibliographicVolumeNumber": "520", "bibliographic_titles": [{"bibliographic_title": "Biochemical and Biophysical Research Communications", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Cis-prenyltransferases are enzymes responsible for the biosynthesis of glycosyl carrier lipids, natural rubber, and some secondary metabolites. Certain organisms, including some archaeal species, possess multiple genes encoding cis-prenyltransferase homologs, and the physiological roles of these seemingly-redundant genes are often obscure. Cis-prenyltransferases usually form homomeric complexes, but recent reports have demonstrated that certain eukaryotic enzymes are heteromeric protein complexes consisting of two homologous subunits. In this study, three cis-prenyltransferase homolog proteins, MM_0014, MM_0618, and MM_1083, from the methanogenic archaeon Methanosarcina mazei are overexpressed in Escherichia coli and partially purified for functional characterization. Coexistence of MM_0618 and MM_1083 exhibits prenyltransferase activity, while each of them alone has almost no activity. The chain-lengths of the products of this heteromeric enzyme are in good agreement with those of glycosyl carrier lipids extracted from M. mazei, which are likely di- and tetra-hydrogenated decaprenyl phosphates, suggesting that the MM_0618/MM_1083 heteromer is involved in glycosyl carrier lipid biosynthesis. MM_0014 acts as a typical homomeric cis-prenyltransferase and produces shorter products.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "ファイル公開：2020-12-03", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.bbrc.2019.09.143", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN（print）", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Emi, Koh-ichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "99129", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sompiyachoke, Kitty", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "99130", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okada, Miyako", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "99131", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hemmi, Hisashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "99132", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "for_institutional_repository.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "for_institutional_repository", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/30061/files/for_institutional_repository.pdf"}, "version_id": "a19d5ae9-5f4a-400d-a091-ad53446e6f19"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Cis-prenyltransferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Archaea", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glycosyl carrier lipid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Heteromer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Isoprenoid", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/00032247", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2020-05-25"}, "publish_date": "2020-05-25", "publish_status": "0", "recid": "30061", "relation": {}, "relation_version_is_last": true, "title": ["A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei"], "weko_shared_id": -1}