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  1. F100 環境医学研究所
  2. F100a 雑誌掲載論文
  3. 学術雑誌

Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair

http://hdl.handle.net/2237/00032522
http://hdl.handle.net/2237/00032522
e6fd2636-8378-454e-87f8-0f09919a479e
名前 / ファイル ライセンス アクション
Cell_mf.pdf Cell_mf (3.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-07-20
タイトル
タイトル Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair
言語 en
著者 Nakazawa, Yuka

× Nakazawa, Yuka

WEKO 100646

en Nakazawa, Yuka

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Hara, Yuichiro

× Hara, Yuichiro

WEKO 100647

en Hara, Yuichiro

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Oka, Yasuyoshi

× Oka, Yasuyoshi

WEKO 100648

en Oka, Yasuyoshi

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Komine, Okiru

× Komine, Okiru

WEKO 100649

en Komine, Okiru

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van den Heuvel, Diana

× van den Heuvel, Diana

WEKO 100650

en van den Heuvel, Diana

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Guo, Chaowan

× Guo, Chaowan

WEKO 100651

en Guo, Chaowan

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Daigaku, Yasukazu

× Daigaku, Yasukazu

WEKO 100652

en Daigaku, Yasukazu

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Isono, Mayu

× Isono, Mayu

WEKO 100653

en Isono, Mayu

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He, Yuxi

× He, Yuxi

WEKO 100654

en He, Yuxi

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Shimada, Mayuko

× Shimada, Mayuko

WEKO 100655

en Shimada, Mayuko

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Kato, Kana

× Kato, Kana

WEKO 100656

en Kato, Kana

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Jia, Nan

× Jia, Nan

WEKO 100657

en Jia, Nan

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Hashimoto, Satoru

× Hashimoto, Satoru

WEKO 100658

en Hashimoto, Satoru

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Kotani, Yuko

× Kotani, Yuko

WEKO 100659

en Kotani, Yuko

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Miyoshi, Yuka

× Miyoshi, Yuka

WEKO 100660

en Miyoshi, Yuka

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Tanaka, Miyako

× Tanaka, Miyako

WEKO 100661

en Tanaka, Miyako

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Sobue, Akira

× Sobue, Akira

WEKO 100662

en Sobue, Akira

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Mitsutake, Norisato

× Mitsutake, Norisato

WEKO 100663

en Mitsutake, Norisato

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Suganami, Takayoshi

× Suganami, Takayoshi

WEKO 100664

en Suganami, Takayoshi

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Masuda, Akio

× Masuda, Akio

WEKO 100665

en Masuda, Akio

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Ohno, Kinji

× Ohno, Kinji

WEKO 100666

en Ohno, Kinji

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Nakada, Shinichiro

× Nakada, Shinichiro

WEKO 100667

en Nakada, Shinichiro

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Mashimo, Tomoji

× Mashimo, Tomoji

WEKO 100668

en Mashimo, Tomoji

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Yamanaka, Koji

× Yamanaka, Koji

WEKO 100669

en Yamanaka, Koji

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Luijsterburg, Martijn S.

× Luijsterburg, Martijn S.

WEKO 100670

en Luijsterburg, Martijn S.

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Ogi, Tomoo

× Ogi, Tomoo

WEKO 100671

en Ogi, Tomoo

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 RNA polymerase II
キーワード
主題Scheme Other
主題 RNAPII
キーワード
主題Scheme Other
主題 ubiquitination of RNAPII
キーワード
主題Scheme Other
主題 transcription coupled repair
キーワード
主題Scheme Other
主題 TCR
キーワード
主題Scheme Other
主題 nucleotide excision repair
キーワード
主題Scheme Other
主題 NER
キーワード
主題Scheme Other
主題 Cockayne syndrome
キーワード
主題Scheme Other
主題 CS
キーワード
主題Scheme Other
主題 UV-sensitive syndrome
キーワード
主題Scheme Other
主題 UVsS
キーワード
主題Scheme Other
主題 ChIP-seq
キーワード
主題Scheme Other
主題 UVSSA
キーワード
主題Scheme Other
主題 CRL/CSA/CSB
キーワード
主題Scheme Other
主題 TFIIH
抄録
内容記述 Transcription-coupled nucleotide excision repair (TC-NER) is initiated by the stalling of elongating RNA polymerase II (RNAPIIo) at DNA lesions. The ubiquitination of RNAPIIo in response to DNA damage is an evolutionarily conserved event, but its function in mammals is unknown. Here, we identified a single DNA damage-induced ubiquitination site in RNAPII at RPB1-K1268, which regulates transcription recovery and DNA damage resistance. Mechanistically, RPB1-K1268 ubiquitination stimulates the association of the core-TFIIH complex with stalled RNAPIIo through a transfer mechanism that also involves UVSSA-K414 ubiquitination. We developed a strand-specific ChIP-seq method, which revealed RPB1-K1268 ubiquitination is important for repair and the resolution of transcriptional bottlenecks at DNA lesions. Finally, RPB1-K1268R knockin mice displayed a short life-span, premature aging, and neurodegeneration. Our results reveal RNAPII ubiquitination provides a two-tier protection mechanism by activating TC-NER and, in parallel, the processing of DNA damage-stalled RNAPIIo, which together prevent prolonged transcription arrest and protect against neurodegeneration.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2021-03-19
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.cell.2020.02.010
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0092-8674
書誌情報 en : Cell

巻 180, 号 6, p. 1228-1244.e24, 発行日 2020-03-19
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