{"_buckets": {"deposit": "21b51a5c-d9b9-4af3-a3ea-59f9aa9646a1"}, "_deposit": {"id": "30421", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "30421"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00030421", "sets": ["2518"]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3", "bibliographicPageEnd": "518", "bibliographicPageStart": "509", "bibliographicVolumeNumber": "82", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Sasa veitchii and other Sasa species are traditional medicinal herbs belonging to a group of Japanese bamboos collectively called Kumazasa, and these species possess the potential for a wide variety of uses. The present study aimed to elucidate the anticancer mechanisms exerted by S. veitchii extract (SE) against a human breast cancer cell line, MCF-7 cells. Freeze-dried Sunchlon® was used as the SE, and cell proliferation activity was measured using the [3H]-thymidine incorporation assay. Induction of apoptosis was assessed via Annexin V and caspase-3 fluorescent staining, the induction of necrosis was measured via propidium iodide staining, and cell cycle-related protein expression was determined using western blot-ting. The IC50 value of the SE was 7.7 μg/mL in MCF-7 cells. Although the primary active ingredient in Sunchlon® is sodium copper chlorophyllin (0.25%), the present results indicated that ingredients other than SCC exert anti-cancer activities (the IC50 value of SCC was 715 μg/mL), and late apoptosis or necrosis was induced in an SE dose-dependent manner. The expression levels of cyclin D1 and Cdk6 were decreased after SE treatment, and there was no change in the Cdk1/2 expression levels. Additionally, the expression of the necrosis-related cell death indicators RIP1 and RIP3 was increased in response to high-dose SE treatments, and this was indicative of cells preparing for programmed cell death. SE induces cell death in MCF-7 cells via the inhibition of cyclin D1 expression at low concentrations, and this extract induces programmed necrosis (necroptosis) by potentiating RIP1/RIP3 expression.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.82.3.509", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University Graduate School of Medicine, School of Medicine"}]}, "item_9_relation_43": {"attribute_name": "異版である", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/823.html ", "subitem_relation_type_select": "URI"}}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN（Online）", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "ISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN（print）", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ichimaru, Yoshimi"}], "nameIdentifiers": [{"nameIdentifier": "101072", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kanaeda, Natsuki"}], "nameIdentifiers": [{"nameIdentifier": "101073", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tominaga, Sarah"}], "nameIdentifiers": [{"nameIdentifier": "101074", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzui, Masumi"}], "nameIdentifiers": [{"nameIdentifier": "101075", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maeda, Tohru"}], "nameIdentifiers": [{"nameIdentifier": "101076", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujii, Hirohisa"}], "nameIdentifiers": [{"nameIdentifier": "101077", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakao, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "101078", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshioka, Hiroki"}], "nameIdentifiers": [{"nameIdentifier": "101079", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-09-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "13_Ichimaru.pdf", "filesize": [{"value": "8.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 8000000.0, "url": {"label": "13_Ichimaru.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/30421/files/13_Ichimaru.pdf"}, "version_id": "2a132004-d320-4da2-a1b1-571070381608"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Sasa veitchii", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cyclin D1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "breast cancer", "subitem_subject_scheme": "Other"}, {"subitem_subject": "sodium copper chlorophyllin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Sasa veitchii extract induces anticancer effects via inhibition of cyclin D1 expression in MCF-7 cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Sasa veitchii extract induces anticancer effects via inhibition of cyclin D1 expression in MCF-7 cells"}]}, "item_type_id": "9", "owner": "1", "path": ["2518"], "permalink_uri": "https://doi.org/10.18999/nagjms.82.3.509", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-09-04"}, "publish_date": "2020-09-04", "publish_status": "0", "recid": "30421", "relation": {}, "relation_version_is_last": true, "title": ["Sasa veitchii extract induces anticancer effects via inhibition of cyclin D1 expression in MCF-7 cells"], "weko_shared_id": null}