{"_buckets": {"deposit": "ca5166ea-fd99-44b6-9aee-391fc6347170"}, "_deposit": {"created_by": 17, "id": "3969", "owners": [17], "pid": {"revision_id": 0, "type": "depid", "value": "3969"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00003969", "sets": []}, "author_link": ["10166", "10167"], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1-2", "bibliographicPageEnd": "44", "bibliographicPageStart": "35", "bibliographicVolumeNumber": "67", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Human neuroblastomas and gliomas express high levels of GD2 ganglioside. Mechanisms for the re-expression of GD2 after the incorporation of an exogenous precursor structure were analyzed using a human heterophilic monoclonal antibody (mAb) together with mouse anti-GD3 and mouse anti-GD2 mAbs. First, mouse anti-GD2 mAb 220-51 was generated and its reactivity was confirmed to be almost identical with that of the well-known mAb 3F8 antibody. As reported previously for GD3 variants, new ganglioside antigens reactive with human mAb 32-27 were analyzed by culturing an astrocytoma cell line AS in the presence of NeuGc-GM3. Analysis of the extracted gangliosides from AS thus cultured revealed a new component detected with mAb 32-27, migrating similarly to GD2. Incorporated NeuGc-GM3 seemed to be converted to NeuAc-NeuGc-type GD3, and then to NeuAc-NeuGc-type GD2 with α2,8-sialyltransferase and β1,4-GalNAc transferase, respectively. In addition, AS was inoculated into nude mice, and glycolipids were extracted from generated tumors. Analysis of the ganglioside components using mAbs indicated that NeuAc-NeuGc-type GD2 was generated in the xenogeneic tumors by incorporating NeuGc-GM3 from mouse blood.  These results indicated the presence of a pathway for utilization of exogenous gangliosides for remodeling and re-expression in vivo.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/5402"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.67.1-2.35", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine", "subitem_publisher_language": "en"}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN（Online）", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "EISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN（print）", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "PISSN"}]}, "item_9_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nishio, Masashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10166", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Furukawa, Koichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10167", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "p35_44.pdf", "filesize": [{"value": "235.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 235100.0, "url": {"label": "p35_44.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/3969/files/p35_44.pdf"}, "version_id": "02d86e44-9f7a-42a4-b7d5-d639426bdafc"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Ganglioside", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NeuG", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Sialic acid", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Recycle", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Glycosyltransferase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Astrocytoma", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "17", "path": ["519"], "permalink_uri": "https://doi.org/10.18999/nagjms.67.1-2.35", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2006-02-10"}, "publish_date": "2006-02-10", "publish_status": "0", "recid": "3969", "relation": {}, "relation_version_is_last": true, "title": ["Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo"], "weko_shared_id": -1}