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Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo
https://doi.org/10.18999/nagjms.67.1-2.35
https://doi.org/10.18999/nagjms.67.1-2.3591371c3f-e9a1-4b12-8609-d31b623dd11d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
p35_44.pdf (235.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-02-10 | |||||
| タイトル | ||||||
| タイトル | Incorporation, remodeling and re-expression of exogenous gangliosides in human cancer cell lines in vitro and in vivo | |||||
| 言語 | en | |||||
| 著者 |
Nishio, Masashi
× Nishio, Masashi× Furukawa, Koichi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ganglioside | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NeuG | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Sialic acid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Recycle | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Glycosyltransferase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Astrocytoma | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Human neuroblastomas and gliomas express high levels of GD2 ganglioside. Mechanisms for the re-expression of GD2 after the incorporation of an exogenous precursor structure were analyzed using a human heterophilic monoclonal antibody (mAb) together with mouse anti-GD3 and mouse anti-GD2 mAbs. First, mouse anti-GD2 mAb 220-51 was generated and its reactivity was confirmed to be almost identical with that of the well-known mAb 3F8 antibody. As reported previously for GD3 variants, new ganglioside antigens reactive with human mAb 32-27 were analyzed by culturing an astrocytoma cell line AS in the presence of NeuGc-GM3. Analysis of the extracted gangliosides from AS thus cultured revealed a new component detected with mAb 32-27, migrating similarly to GD2. Incorporated NeuGc-GM3 seemed to be converted to NeuAc-NeuGc-type GD3, and then to NeuAc-NeuGc-type GD2 with α2,8-sialyltransferase and β1,4-GalNAc transferase, respectively. In addition, AS was inoculated into nude mice, and glycolipids were extracted from generated tumors. Analysis of the ganglioside components using mAbs indicated that NeuAc-NeuGc-type GD2 was generated in the xenogeneic tumors by incorporating NeuGc-GM3 from mouse blood. These results indicated the presence of a pathway for utilization of exogenous gangliosides for remodeling and re-expression in vivo. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.67.1-2.35 | |||||
| ID登録タイプ | JaLC | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 67, 号 1-2, p. 35-44, 発行日 2004-05 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/5402 | |||||
| 識別子タイプ | HDL | |||||
