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高等植物の細胞増殖因子に関する生物有機化学的研究
http://hdl.handle.net/2237/6441
7a5dec6a-0969-4662-abd8-d76358a241c3
名前 / ファイル | ライセンス | アクション | |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2006-05-30 | |||||
タイトル | ||||||
タイトル | 高等植物の細胞増殖因子に関する生物有機化学的研究 | |||||
著者 |
松林, 嘉克
× 松林, 嘉克× Matsubayashi, Yoshikatsu |
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抄録 | ||||||
内容記述 | Peptidal growth factors were isolated from conditioned medium derived from rice (Oriza sativa L.) suspension cultures and identified to be a sulfated pentapeptide(Htyr (SO3H)-Ile-Tyr (SO3H)-Thr-Gln-OH)and its C-terminal truncated tetrapeptide (Htyr (SO3H)-Ile-Tyr(SO3H)-Thr-OH).These structures were identical to the phytosulfokines originally found in asparagus (Asparagus officinalis L.) mesophyll cultures. The pentapeptide phytosulfokine-α(PSK-α) very storongly stimulated colony formation of rice protoplasts at concentrations above 10-8 M, indicating a species-independent mode of action of phytosulfokines. Binding assays using 35S-labeled phytosulfoking sites on the surface of rice cells in susupension. Analysis of [35S]PSK-α binding in differential centrifugation fractions suggested association of the binding with a plasma membrane -enriched fraction. The apparent Kd values for[35S]PSK-αbinding was found to be 1 x 10-9 M for the high affinity type and 1 x 10-7 M for the low affinty type, with maximum numbers of binding sites of 1 x 104 sites/cell and 1 x 105 sites/cell, respectively. Competition studies with the [35S]PSK-αand several synthetic PSK-αanalogs demonstrated that only peptides which possesses mitogenic activity can effectively displace the radioligand. These results suggest that a novel signal transduction pathway mediated by peptidal factors is involved in plant cell proliferation. | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 名古屋大学博士学位論文 学位の種類:博士(農学) (課程) 学位授与年月日:平成9年10月31日 | |||||
内容記述タイプ | Other | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
書誌情報 | 発行日 1997-10-31 | |||||
学位授与年度 | ||||||
学位授与年度 | 1997 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1997-10-31 | |||||
学位授与番号 | ||||||
学位授与番号 | 13901甲第3831号 | |||||
フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/6441 | |||||
識別子タイプ | HDL |