{"_buckets": {"deposit": "f7134ecd-6409-4005-b124-3592ecb74095"}, "_deposit": {"id": "4905", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "4905"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00004905"}, "item_12_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1997-10-31", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_12_date_granted_64": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "1997-10-31"}]}, "item_12_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Peptidal growth factors were isolated from conditioned medium derived from rice (Oriza sativa L.) suspension cultures and identified to be a sulfated pentapeptide(Htyr (SO3H)-Ile-Tyr (SO3H)-Thr-Gln-OH\uff09and its C-terminal truncated tetrapeptide (Htyr (SO3H)-Ile-Tyr(SO3H)-Thr-OH).These structures were identical to the phytosulfokines originally found in asparagus (Asparagus officinalis L.) mesophyll cultures. The pentapeptide phytosulfokine-\u03b1(PSK-\u03b1) very storongly stimulated colony formation of rice protoplasts at concentrations above 10-8 M, indicating a species-independent mode of action of phytosulfokines. Binding assays using 35S-labeled phytosulfoking sites on the surface of rice cells in susupension. Analysis of [35S]PSK-\u03b1 binding in differential centrifugation fractions suggested association of the binding with a plasma membrane -enriched fraction. The apparent Kd values for[35S]PSK-\u03b1binding was found to be 1 x 10-9 M for the high affinity type and 1 x 10-7 M for the low affinty type, with maximum numbers of binding sites of 1 x 104 sites/cell and 1 x 105 sites/cell, respectively. Competition studies with the [35S]PSK-\u03b1and several synthetic PSK-\u03b1analogs demonstrated that only peptides which possesses mitogenic activity can effectively displace the radioligand. These results suggest that a novel signal transduction pathway mediated by peptidal factors is involved in plant cell proliferation.", "subitem_description_type": "Abstract"}]}, "item_12_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u540d\u53e4\u5c4b\u5927\u5b66\u535a\u58eb\u5b66\u4f4d\u8ad6\u6587 \u5b66\u4f4d\u306e\u7a2e\u985e:\u535a\u58eb(\u8fb2\u5b66) (\u8ab2\u7a0b) \u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5:\u5e73\u62109\u5e7410\u670831\u65e5", "subitem_description_type": "Other"}]}, "item_12_dissertation_number_65": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13901\u7532\u7b2c3831\u53f7"}]}, "item_12_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/6441"}]}, "item_12_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_12_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_12_text_63": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u5ea6", "attribute_value_mlt": [{"subitem_text_value": "1997"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u677e\u6797, \u5609\u514b"}], "nameIdentifiers": [{"nameIdentifier": "12223", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsubayashi, Yoshikatsu"}], "nameIdentifiers": [{"nameIdentifier": "12224", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ko3831.pdf", "filesize": [{"value": "5.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 5900000.0, "url": {"label": "ko3831.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/4905/files/ko3831.pdf"}, "version_id": "d4db4426-061d-4245-b433-1d8d25f76306"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "\u9ad8\u7b49\u690d\u7269\u306e\u7d30\u80de\u5897\u6b96\u56e0\u5b50\u306b\u95a2\u3059\u308b\u751f\u7269\u6709\u6a5f\u5316\u5b66\u7684\u7814\u7a76", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u9ad8\u7b49\u690d\u7269\u306e\u7d30\u80de\u5897\u6b96\u56e0\u5b50\u306b\u95a2\u3059\u308b\u751f\u7269\u6709\u6a5f\u5316\u5b66\u7684\u7814\u7a76"}]}, "item_type_id": "12", "owner": "1", "path": ["643/644/645"], "permalink_uri": "http://hdl.handle.net/2237/6441", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2006-05-30"}, "publish_date": "2006-05-30", "publish_status": "0", "recid": "4905", "relation": {}, "relation_version_is_last": true, "title": ["\u9ad8\u7b49\u690d\u7269\u306e\u7d30\u80de\u5897\u6b96\u56e0\u5b50\u306b\u95a2\u3059\u308b\u751f\u7269\u6709\u6a5f\u5316\u5b66\u7684\u7814\u7a76"], "weko_shared_id": null}