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  1. B300 農学部/生命農学研究科
  2. B300d 学位論文
  3. 博士論文(農博・論農博)

高等植物の細胞増殖因子に関する生物有機化学的研究

http://hdl.handle.net/2237/6441
http://hdl.handle.net/2237/6441
7a5dec6a-0969-4662-abd8-d76358a241c3
名前 / ファイル ライセンス アクション
ko3831.pdf ko3831.pdf (5.9 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2006-05-30
タイトル
タイトル 高等植物の細胞増殖因子に関する生物有機化学的研究
言語 ja
著者 松林, 嘉克

× 松林, 嘉克

WEKO 12223

ja 松林, 嘉克

Search repository
Matsubayashi, Yoshikatsu

× Matsubayashi, Yoshikatsu

WEKO 12224

en Matsubayashi, Yoshikatsu

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
抄録
内容記述 Peptidal growth factors were isolated from conditioned medium derived from rice (Oriza sativa L.) suspension cultures and identified to be a sulfated pentapeptide(Htyr (SO3H)-Ile-Tyr (SO3H)-Thr-Gln-OH)and its C-terminal truncated tetrapeptide (Htyr (SO3H)-Ile-Tyr(SO3H)-Thr-OH).These structures were identical to the phytosulfokines originally found in asparagus (Asparagus officinalis L.) mesophyll cultures. The pentapeptide phytosulfokine-α(PSK-α) very storongly stimulated colony formation of rice protoplasts at concentrations above 10-8 M, indicating a species-independent mode of action of phytosulfokines. Binding assays using 35S-labeled phytosulfoking sites on the surface of rice cells in susupension. Analysis of [35S]PSK-α binding in differential centrifugation fractions suggested association of the binding with a plasma membrane -enriched fraction. The apparent Kd values for[35S]PSK-αbinding was found to be 1 x 10-9 M for the high affinity type and 1 x 10-7 M for the low affinty type, with maximum numbers of binding sites of 1 x 104 sites/cell and 1 x 105 sites/cell, respectively. Competition studies with the [35S]PSK-αand several synthetic PSK-αanalogs demonstrated that only peptides which possesses mitogenic activity can effectively displace the radioligand. These results suggest that a novel signal transduction pathway mediated by peptidal factors is involved in plant cell proliferation.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 名古屋大学博士学位論文 学位の種類:博士(農学) (課程) 学位授与年月日:平成9年10月31日
言語 ja
内容記述タイプ Other
言語
言語 jpn
資源タイプ
資源 http://purl.org/coar/resource_type/c_db06
タイプ doctoral thesis
書誌情報
発行日 1997-10-31
学位名
言語 ja
学位名 博士(農学)
学位授与機関
学位授与機関識別子Scheme kakenhi
学位授与機関識別子 13901
言語 ja
学位授与機関名 名古屋大学
言語 en
学位授与機関名 NAGOYA University
学位授与年度
学位授与年度 1997
学位授与年月日
学位授与年月日 1997-10-31
学位授与番号
学位授与番号 甲第3831号
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/6441
識別子タイプ HDL
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