{"_buckets": {"deposit": "22d2fff1-e09a-4375-bec7-90adfb5ce086"}, "_deposit": {"id": "5481", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5481"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00005481"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2004-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "819", "bibliographicPageStart": "812", "bibliographicVolumeNumber": "279", "bibliographic_titles": [{"bibliographic_title": "Journal of Biological Chemistry"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "In plants, the cation/H^+ exchanger translocates Ca^{2+}and other metal ions into vacuoles using the H^+ gradient formed by H^+-ATPase and H^+-pyrophosphatase. Such exchangers carrying 11 transmembrane domains have been isolated from plants, yeast and bacteria. In this study, multiple sequence alignment of several cation/H^+ exchangers revealed the presence of 36 residue highly conserved regions between the 3rd and 4th, and the 8th and 9th transmembrane domains. These two repetitive motifs are designated repeats c-1 and c-2. Using site-directed mutagenesis, we generated 31 mutations in the repeats of the Oryza sativa cation/H^+ exchanger, which translocates Ca^{2+} and Mn^{2+}. Mutant exchangers were expressed in a Saccharomyces cerevisiae strain that was sensitive to Ca^{2+} and Mn^{2+} due to the absence of vacuolar Ca^{2+}-ATPase and the Ca^{2+}/H+ exchanger. Mutant exchangers were classified into 6 classes according to their tolerance to Ca^{2+ }and Mn^{2+}. For example, the class III mutants had no tolerance to either ion and the class IV mutants had tolerance only to Ca^{2+}. The biochemical function of each residue was estimated. We investigated the membrane topology of the repeats using a method combining cysteine mutagenesis with sulfhydryl reagents. Our results suggest that the repeat c-1 re-enters the membrane from the vacuolar lumen side and forms a solution-accessible region. Furthermore, several residues in repeats c-1 and c-2 were found to be conserved in animal Na+/Ca^{2+} exchangers. Finally, we suggest that these re-entrant repeats may form a vestibule or filter for cation selection.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/7088"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "the American Society for Biochemistry and Molecular Biology"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://dx.doi.org/10.1074/jbc.M309726200", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "ISSN"}]}, "item_10_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kamiya, Takehiro"}], "nameIdentifiers": [{"nameIdentifier": "14139", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maeshima, Masayoshi"}], "nameIdentifiers": [{"nameIdentifier": "14140", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2004-JBC-OsCAX.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "2004-JBC-OsCAX.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/5481/files/2004-JBC-OsCAX.pdf"}, "version_id": "24eebb3a-d4cf-4c38-8c23-e473a9b181f1"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/7088", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2006-10-27"}, "publish_date": "2006-10-27", "publish_status": "0", "recid": "5481", "relation": {}, "relation_version_is_last": true, "title": ["Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations"], "weko_shared_id": 3}