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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations

http://hdl.handle.net/2237/7088
http://hdl.handle.net/2237/7088
b1b01349-56c0-444e-b202-01f7cc070753
名前 / ファイル ライセンス アクション
2004-JBC-OsCAX.pdf 2004-JBC-OsCAX.pdf (1.1 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2006-10-27
タイトル
タイトル Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations
言語 en
著者 Kamiya, Takehiro

× Kamiya, Takehiro

WEKO 14139

en Kamiya, Takehiro

Search repository
Maeshima, Masayoshi

× Maeshima, Masayoshi

WEKO 14140

en Maeshima, Masayoshi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
抄録
内容記述 In plants, the cation/H^+ exchanger translocates Ca^{2+}and other metal ions into vacuoles using the H^+ gradient formed by H^+-ATPase and H^+-pyrophosphatase. Such exchangers carrying 11 transmembrane domains have been isolated from plants, yeast and bacteria. In this study, multiple sequence alignment of several cation/H^+ exchangers revealed the presence of 36 residue highly conserved regions between the 3rd and 4th, and the 8th and 9th transmembrane domains. These two repetitive motifs are designated repeats c-1 and c-2. Using site-directed mutagenesis, we generated 31 mutations in the repeats of the Oryza sativa cation/H^+ exchanger, which translocates Ca^{2+} and Mn^{2+}. Mutant exchangers were expressed in a Saccharomyces cerevisiae strain that was sensitive to Ca^{2+} and Mn^{2+} due to the absence of vacuolar Ca^{2+}-ATPase and the Ca^{2+}/H+ exchanger. Mutant exchangers were classified into 6 classes according to their tolerance to Ca^{2+ }and Mn^{2+}. For example, the class III mutants had no tolerance to either ion and the class IV mutants had tolerance only to Ca^{2+}. The biochemical function of each residue was estimated. We investigated the membrane topology of the repeats using a method combining cysteine mutagenesis with sulfhydryl reagents. Our results suggest that the repeat c-1 re-enters the membrane from the vacuolar lumen side and forms a solution-accessible region. Furthermore, several residues in repeats c-1 and c-2 were found to be conserved in animal Na+/Ca^{2+} exchangers. Finally, we suggest that these re-entrant repeats may form a vestibule or filter for cation selection.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 the American Society for Biochemistry and Molecular Biology
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1074/jbc.M309726200
ISSN
収録物識別子タイプ PISSN
収録物識別子 0021-9258
書誌情報 en : Journal of Biological Chemistry

巻 279, 号 1, p. 812-819, 発行日 2004-01
フォーマット
application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/7088
識別子タイプ HDL
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