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Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations
http://hdl.handle.net/2237/7088
http://hdl.handle.net/2237/7088b1b01349-56c0-444e-b202-01f7cc070753
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2004-JBC-OsCAX.pdf (1.1 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2006-10-27 | |||||
| タイトル | ||||||
| タイトル | Residues in Internal Repeats of the Rice Cation/H+ Exchanger are Involved in the Transport and Selection of Cations | |||||
| 言語 | en | |||||
| 著者 |
Kamiya, Takehiro
× Kamiya, Takehiro× Maeshima, Masayoshi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In plants, the cation/H^+ exchanger translocates Ca^{2+}and other metal ions into vacuoles using the H^+ gradient formed by H^+-ATPase and H^+-pyrophosphatase. Such exchangers carrying 11 transmembrane domains have been isolated from plants, yeast and bacteria. In this study, multiple sequence alignment of several cation/H^+ exchangers revealed the presence of 36 residue highly conserved regions between the 3rd and 4th, and the 8th and 9th transmembrane domains. These two repetitive motifs are designated repeats c-1 and c-2. Using site-directed mutagenesis, we generated 31 mutations in the repeats of the Oryza sativa cation/H^+ exchanger, which translocates Ca^{2+} and Mn^{2+}. Mutant exchangers were expressed in a Saccharomyces cerevisiae strain that was sensitive to Ca^{2+} and Mn^{2+} due to the absence of vacuolar Ca^{2+}-ATPase and the Ca^{2+}/H+ exchanger. Mutant exchangers were classified into 6 classes according to their tolerance to Ca^{2+ }and Mn^{2+}. For example, the class III mutants had no tolerance to either ion and the class IV mutants had tolerance only to Ca^{2+}. The biochemical function of each residue was estimated. We investigated the membrane topology of the repeats using a method combining cysteine mutagenesis with sulfhydryl reagents. Our results suggest that the repeat c-1 re-enters the membrane from the vacuolar lumen side and forms a solution-accessible region. Furthermore, several residues in repeats c-1 and c-2 were found to be conserved in animal Na+/Ca^{2+} exchangers. Finally, we suggest that these re-entrant repeats may form a vestibule or filter for cation selection. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | the American Society for Biochemistry and Molecular Biology | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1074/jbc.M309726200 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0021-9258 | |||||
| 書誌情報 |
en : Journal of Biological Chemistry 巻 279, 号 1, p. 812-819, 発行日 2004-01 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/7088 | |||||
| 識別子タイプ | HDL | |||||
