{"_buckets": {"deposit": "37114338-fa69-41f9-862a-ec8894def859"}, "_deposit": {"id": "5831", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5831"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00005831", "sets": ["501"]}, "author_link": ["15488", "15489", "15490", "15491", "15492"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "21", "bibliographicPageEnd": "19197", "bibliographicPageStart": "19191", "bibliographicVolumeNumber": "277", "bibliographic_titles": [{"bibliographic_title": "Journal of Biological Chemistry", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Interstitial cells of Cajal (ICC) are considered to be pacemaker cells in gastrointestinal tracts. ICC generate rhythmical electronic activity as slow waves, which is dihydropyridine-insensitive, and drive spontaneous contraction of smooth muscles. Although cytosolic Ca2+ has been assumed to play a key role in pacemaking, Ca2+ movements in ICC have not yet been examined in detail. In the present study, using cultured cell clusters isolated from the mouse small intestine, we demonstrated Ca2+ oscillations in ICC. Fluo4 was loaded to the cell cluster, the relative amount of cytosolic Ca2+ was recorded, and ICC were identified by c-Kit immunoreactivity. We specifically detected Ca2+ oscillation in ICC in the presence of dihydropyridine, which abolishes Ca2+ oscillation in smooth muscles. The oscillation was coupled to the electrical activity corresponding to slow waves and depended on Ca2+ influx through a non-selective cation channel, which was SK\u0026F 96365-sensitive and store-operated. We further demonstrated the presence of transient receptor potential-like channel 4 (TRP4) in caveolae of ICC. Taken together, the results infer that the Ca2+ oscillation in ICC is intimately linked to the pacemaker function and depends on Ca2+ influx mediated by TRP4.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/7447"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "The American Society for Biochemistry and Molecular Biology", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1074/jbc.M201728200", "subitem_relation_type_select": "DOI"}}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021-9258", "subitem_source_identifier_type": "PISSN"}]}, "item_10_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Torihashi, Shigeko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15488", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Fujimoto, Toyoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15489", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Trost, Claudia", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15490", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakayama, Shinsuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "15491", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "鳥橋, 茂子", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "15492", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "M2-01728a.pdf", "filesize": [{"value": "1.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 1100000.0, "url": {"label": "M2-01728a.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/5831/files/M2-01728a.pdf"}, "version_id": "712d588d-9131-4994-9feb-970f576a3e34"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["501"], "permalink_uri": "http://hdl.handle.net/2237/7447", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-03-08"}, "publish_date": "2007-03-08", "publish_status": "0", "recid": "5831", "relation": {}, "relation_version_is_last": true, "title": ["Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae"], "weko_shared_id": -1}