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Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae
http://hdl.handle.net/2237/7447
http://hdl.handle.net/2237/744778672df5-d126-404d-9b00-4a4f287db265
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-03-08 | |||||
タイトル | ||||||
タイトル | Calcium oscillation linked to pacemaking of interstitial cells of Cajal;Requirement of calcium influx and localisation of TRP4 in caveolae | |||||
言語 | en | |||||
著者 |
Torihashi, Shigeko
× Torihashi, Shigeko× Fujimoto, Toyoshi× Trost, Claudia× Nakayama, Shinsuke× 鳥橋, 茂子 |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述 | Interstitial cells of Cajal (ICC) are considered to be pacemaker cells in gastrointestinal tracts. ICC generate rhythmical electronic activity as slow waves, which is dihydropyridine-insensitive, and drive spontaneous contraction of smooth muscles. Although cytosolic Ca2+ has been assumed to play a key role in pacemaking, Ca2+ movements in ICC have not yet been examined in detail. In the present study, using cultured cell clusters isolated from the mouse small intestine, we demonstrated Ca2+ oscillations in ICC. Fluo4 was loaded to the cell cluster, the relative amount of cytosolic Ca2+ was recorded, and ICC were identified by c-Kit immunoreactivity. We specifically detected Ca2+ oscillation in ICC in the presence of dihydropyridine, which abolishes Ca2+ oscillation in smooth muscles. The oscillation was coupled to the electrical activity corresponding to slow waves and depended on Ca2+ influx through a non-selective cation channel, which was SK&F 96365-sensitive and store-operated. We further demonstrated the presence of transient receptor potential-like channel 4 (TRP4) in caveolae of ICC. Taken together, the results infer that the Ca2+ oscillation in ICC is intimately linked to the pacemaker function and depends on Ca2+ influx mediated by TRP4. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
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言語 | en | |||||
出版者 | The American Society for Biochemistry and Molecular Biology | |||||
言語 | ||||||
言語 | eng | |||||
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資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
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出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1074/jbc.M201728200 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0021-9258 | |||||
書誌情報 |
en : Journal of Biological Chemistry 巻 277, 号 21, p. 19191-19197, 発行日 2002-05 |
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application/pdf | ||||||
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値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/7447 | |||||
識別子タイプ | HDL |