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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail

http://hdl.handle.net/2237/9265
http://hdl.handle.net/2237/9265
e6469c26-15ac-4aeb-889e-e27c073a24a5
名前 / ファイル ライセンス アクション
2-16-04SETAC-Review.pdf 2-16-04SETAC-Review.pdf (276.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-01-10
タイトル
タイトル In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail
言語 en
著者 Shimada, Kiyoshi

× Shimada, Kiyoshi

WEKO 21311

en Shimada, Kiyoshi

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Ha, Yonju

× Ha, Yonju

WEKO 21312

en Ha, Yonju

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Tsukada, Akira

× Tsukada, Akira

WEKO 21313

en Tsukada, Akira

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Saito, Noboru

× Saito, Noboru

WEKO 21314

en Saito, Noboru

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Maekawa, Shinobu

× Maekawa, Shinobu

WEKO 21315

en Maekawa, Shinobu

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Nishizuka, Makoto

× Nishizuka, Makoto

WEKO 21316

en Nishizuka, Makoto

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Imagawa, Masayoshi

× Imagawa, Masayoshi

WEKO 21317

en Imagawa, Masayoshi

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アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
キーワード
主題Scheme Other
主題 endocrine disrupters
キーワード
主題Scheme Other
主題 estrogen receptor
キーワード
主題Scheme Other
主題 competitive enzyme immunoassay
抄録
内容記述タイプ Abstract
内容記述 This review paper describes (1) establishment of an in vitro method for screening endocrine disrupting chemicals with estrogenic activity and (2) study on effects of nonylphenol (NP) and octylphenol (OP) on mRNA expression of vitellogenin (VTG) Ⅱ and very low density lipoprotein (apoVLDL) Ⅱ in the liver of quail embryos. For in vitro screening, the cDNA of the ligand binding domain of quail estrogen receptor (ER) α or quail β was ligated into the gluthathione S-transferase expression vector and expressed in E.coli. The fusion protein was used for competitive enzyme immunoassay. Among 20 substances examined, 9 and 5 showed binding to ER α and β, respectively. NP and OP showed the highest binding to ER α, and bisphenol-A to ER β, with binding affinities relative to that of diethylstilbesterol (10^{-9}M order) of 6 % and 16.2 %, respectively. For the in vivo study, quail fertile eggs were treated with a single injection of either NP or OP(10 and 100 nmole/egg, in 20 μl) on day 13 of incubation. VTGⅡ and apo VLDLⅡ mRNA levels on day 15 of incubation were determined by RT-PCR. A weak but distinct expression of VTG Ⅱ and ApoVLDLⅡmRNA was observed after NP and OP. NP and OP could be endocrine disruptors in birds.
言語 en
出版者
出版者 Science Reviews 2000 Ltd
言語 en
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
ISSN
収録物識別子タイプ PISSN
収録物識別子 1470-2061
書誌情報 en : Avian and Poultry Biology Reviews

巻 16, p. 19-27, 発行日 2005
フォーマット
値 application/pdf
著者版フラグ
値 publisher
URI
識別子 http://hdl.handle.net/2237/9265
識別子タイプ HDL
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