{"_buckets": {"deposit": "2fc072f3-88e4-4cbc-b85d-230d339888cc"}, "_deposit": {"id": "7567", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "7567"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00007567"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "27", "bibliographicPageStart": "19", "bibliographicVolumeNumber": "16", "bibliographic_titles": [{"bibliographic_title": "Avian and Poultry Biology Reviews"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "This review paper describes (1) establishment of an in vitro method for screening endocrine disrupting chemicals with estrogenic activity and (2) study on effects of nonylphenol (NP) and octylphenol (OP) on mRNA expression of vitellogenin (VTG) \u2161 and very low density lipoprotein (apoVLDL) \u2161 in the liver of quail embryos. For in vitro screening, the cDNA of the ligand binding domain of quail estrogen receptor (ER) \u03b1 or quail \u03b2 was ligated into the gluthathione S-transferase expression vector and expressed in E.coli. The fusion protein was used for competitive enzyme immunoassay. Among 20 substances examined, 9 and 5 showed binding to ER \u03b1 and \u03b2, respectively. NP and OP showed the highest binding to ER \u03b1, and bisphenol-A to ER \u03b2, with binding affinities relative to that of diethylstilbesterol (10^{-9}M order) of 6 \uff05 and 16.2 \uff05, respectively. For the in vivo study, quail fertile eggs were treated with a single injection of either NP or OP(10 and 100 nmole/egg, in 20 \u03bcl) on day 13 of incubation. VTG\u2161 and apo VLDL\u2161 mRNA levels on day 15 of incubation were determined by RT-PCR. A weak but distinct expression of VTG \u2161 and ApoVLDL\u2161mRNA was observed after NP and OP. NP and OP could be endocrine disruptors in birds. \u3000", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/9265"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Science Reviews 2000 Ltd"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1470-2061", "subitem_source_identifier_type": "ISSN"}]}, "item_10_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shimada, Kiyoshi"}], "nameIdentifiers": [{"nameIdentifier": "21311", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ha, Yonju"}], "nameIdentifiers": [{"nameIdentifier": "21312", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsukada, Akira"}], "nameIdentifiers": [{"nameIdentifier": "21313", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Saito, Noboru"}], "nameIdentifiers": [{"nameIdentifier": "21314", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maekawa, Shinobu"}], "nameIdentifiers": [{"nameIdentifier": "21315", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishizuka, Makoto"}], "nameIdentifiers": [{"nameIdentifier": "21316", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imagawa, Masayoshi"}], "nameIdentifiers": [{"nameIdentifier": "21317", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2-16-04SETAC-Review.pdf", "filesize": [{"value": "276.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 276600.0, "url": {"label": "2-16-04SETAC-Review.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/7567/files/2-16-04SETAC-Review.pdf"}, "version_id": "a7e99fc2-dad1-4ed3-8a1e-66b176105ad9"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "endocrine disrupters", "subitem_subject_scheme": "Other"}, {"subitem_subject": "estrogen receptor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "competitive enzyme immunoassay", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/9265", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2008-01-10"}, "publish_date": "2008-01-10", "publish_status": "0", "recid": "7567", "relation": {}, "relation_version_is_last": true, "title": ["In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail"], "weko_shared_id": null}