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In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail
http://hdl.handle.net/2237/9265
http://hdl.handle.net/2237/9265e6469c26-15ac-4aeb-889e-e27c073a24a5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2-16-04SETAC-Review.pdf (276.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-01-10 | |||||
| タイトル | ||||||
| タイトル | In vivo and in vitro screening of endocrine disrupting chemicals with estrogenic activity in Japanese quail | |||||
| 言語 | en | |||||
| 著者 |
Shimada, Kiyoshi
× Shimada, Kiyoshi× Ha, Yonju× Tsukada, Akira× Saito, Noboru× Maekawa, Shinobu× Nishizuka, Makoto× Imagawa, Masayoshi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | endocrine disrupters | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | estrogen receptor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | competitive enzyme immunoassay | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | This review paper describes (1) establishment of an in vitro method for screening endocrine disrupting chemicals with estrogenic activity and (2) study on effects of nonylphenol (NP) and octylphenol (OP) on mRNA expression of vitellogenin (VTG) Ⅱ and very low density lipoprotein (apoVLDL) Ⅱ in the liver of quail embryos. For in vitro screening, the cDNA of the ligand binding domain of quail estrogen receptor (ER) α or quail β was ligated into the gluthathione S-transferase expression vector and expressed in E.coli. The fusion protein was used for competitive enzyme immunoassay. Among 20 substances examined, 9 and 5 showed binding to ER α and β, respectively. NP and OP showed the highest binding to ER α, and bisphenol-A to ER β, with binding affinities relative to that of diethylstilbesterol (10^{-9}M order) of 6 ％ and 16.2 ％, respectively. For the in vivo study, quail fertile eggs were treated with a single injection of either NP or OP(10 and 100 nmole/egg, in 20 μl) on day 13 of incubation. VTGⅡ and apo VLDLⅡ mRNA levels on day 15 of incubation were determined by RT-PCR. A weak but distinct expression of VTG Ⅱ and ApoVLDLⅡmRNA was observed after NP and OP. NP and OP could be endocrine disruptors in birds. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Science Reviews 2000 Ltd | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1470-2061 | |||||
| 書誌情報 |
en : Avian and Poultry Biology Reviews 巻 16, p. 19-27, 発行日 2005 |
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| フォーマット | ||||||
| 値 | application/pdf | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/9265 | |||||
| 識別子タイプ | HDL | |||||
