{"_buckets": {"deposit": "6d4f5b47-c996-4cad-bec9-bc4bf86e2ea1"}, "_deposit": {"id": "1373", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1373"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00001373"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1/2", "bibliographicPageEnd": "57", "bibliographicPageStart": "55", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "ZAKI-4 inhibits the activity of calcineurin, a Ca^\u003c2+\u003e-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 \u03b1 mRNA was mainly expressed in brain whereas ZAKI-4 \u03b2 mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 \u03b2 knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 \u03b2 sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 \u03b2 locus. These clones will be used to establish the knock out mice.", "subitem_description_type": "Abstract"}]}, "item_9_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u56fd\u7acb\u60c5\u5831\u5b66\u7814\u7a76\u6240\u3067\u96fb\u5b50\u5316\u3057\u305f\u30b3\u30f3\u30c6\u30f3\u30c4\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u3002", "subitem_description_type": "Other"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/2783"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/envm.46.55", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Research Institute of Environmental Medicine, Nagoya University"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "02870517", "subitem_source_identifier_type": "ISSN"}]}, "item_9_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KANOU, Yasuhiko"}], "nameIdentifiers": [{"nameIdentifier": "3129", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ABE, Naoki"}], "nameIdentifiers": [{"nameIdentifier": "3130", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ISHIDA, Junji"}], "nameIdentifiers": [{"nameIdentifier": "3131", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "FUKAMIZU, Akiyoshi"}], "nameIdentifiers": [{"nameIdentifier": "3132", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SEO, Hisao"}], "nameIdentifiers": [{"nameIdentifier": "3133", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MURATA, Yoshiharu"}], "nameIdentifiers": [{"nameIdentifier": "3134", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00000034863.pdf", "filesize": [{"value": "430.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 430900.0, "url": {"label": "KJ00000034863.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/1373/files/KJ00000034863.pdf"}, "version_id": "e86aca93-3818-42da-b86b-06b8f58802b5"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "ZAKI-4", "subitem_subject_scheme": "Other"}, {"subitem_subject": "homologous recombination", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gene targeting", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcineurin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression"}]}, "item_type_id": "9", "owner": "1", "path": ["329/333/334/335"], "permalink_uri": "https://doi.org/10.18999/envm.46.55", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2006-01-05"}, "publish_date": "2006-01-05", "publish_status": "0", "recid": "1373", "relation": {}, "relation_version_is_last": true, "title": ["Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression"], "weko_shared_id": null}