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Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression
https://doi.org/10.18999/envm.46.55
https://doi.org/10.18999/envm.46.55310bd83a-9c4a-4b52-8e45-eb6dad6902e7
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-01-05 | |||||
タイトル | ||||||
タイトル | Homologous Recombination of Mouse ZAKI-4 Gene to Disrupt its Expression | |||||
言語 | en | |||||
著者 |
KANOU, Yasuhiko
× KANOU, Yasuhiko× ABE, Naoki× ISHIDA, Junji× FUKAMIZU, Akiyoshi× SEO, Hisao× MURATA, Yoshiharu |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ZAKI-4 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | homologous recombination | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gene targeting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | calcineurin | |||||
抄録 | ||||||
内容記述 | ZAKI-4 inhibits the activity of calcineurin, a Ca^<2+>-dependent protein phosphatase. From ZAKI-4 gene, two isoforms, a and P are generated by an alternative splicing. In adult mice ZAKI-4 α mRNA was mainly expressed in brain whereas ZAKI-4 β mRNA wasubiquitously. To elucidate the specific function of ZAKI-4 isoforms, we plan to establish ZAKI-4 β knock out mice by homologousrecombination. For this purpose, mouse embryonic stem cells were electroporated with a targeting vector in which ZAKI-4 β sequencewas disrupted by cDNA coding neomycin resistance. Six independent clones out of 466 antibiotics-resistant colonies underwenthomologous recombination at the ZAKI-4 β locus. These clones will be used to establish the knock out mice. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | 国立情報学研究所で電子化したコンテンツを使用している。 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Research Institute of Environmental Medicine, Nagoya University | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
ID登録 | ||||||
ID登録 | 10.18999/envm.46.55 | |||||
ID登録タイプ | JaLC | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 02870517 | |||||
書誌情報 |
en : Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 巻 46, 号 1/2, p. 55-57, 発行日 2002-12 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/2783 | |||||
識別子タイプ | HDL |