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  1. C100 医学部/医学系研究科
  2. C100b 紀要
  3. Nagoya journal of medical science
  4. 57(3-4)

EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS

https://doi.org/10.18999/nagjms.57.3-4.119
https://doi.org/10.18999/nagjms.57.3-4.119
cb47ec8c-492a-4d53-9edd-5bec1507a521
名前 / ファイル ライセンス アクション
v57n34p119_126.pdf v57n34p119_126.pdf (557.6 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2012-03-06
タイトル
タイトル EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS
著者 YAMAMOTO, MASANORI

× YAMAMOTO, MASANORI

WEKO 98926

YAMAMOTO, MASANORI

Search repository
HIBI, HATSUKI

× HIBI, HATSUKI

WEKO 98927

HIBI, HATSUKI

Search repository
TSUJI, YOSHIKAZU

× TSUJI, YOSHIKAZU

WEKO 98928

TSUJI, YOSHIKAZU

Search repository
MIYAKE, KOJI

× MIYAKE, KOJI

WEKO 98929

MIYAKE, KOJI

Search repository
キーワード
主題Scheme Other
主題 Androgen
キーワード
主題Scheme Other
主題 Epididymis
キーワード
主題Scheme Other
主題 Cytoplasmic receptor
キーワード
主題Scheme Other
主題 Methionine
キーワード
主題Scheme Other
主題 Cycloheximide
キーワード
主題Scheme Other
主題 Nocodazole
抄録
内容記述 The effects of protein synthesis inhibition and disassembly of microtubules in the epididymal epithelia on proluminal movement of 3H-androgens were investigated by using in vivo microperifusion of 3H-testosterone and subsequent micropuncture to obtain peritubular and intraluminal fluids of caput epididymal tubules. Cycloheximide (100 μg/ml) was used as protein synthesis inhibitor. Nocodazole (3 μg/ml) was used to depolymerize microtubules in the cell. The perifusion fluid was Minimum Essential Medium containing 26.7 μCi/ml 3H-testosterone and 1.3 μCi/ml 14C-polyethyleneglycol (14C-PEG), or the same fluid supplemented with cycloheximide or nocodazole. Radioactivity of 3H-androgen and 14C-PEG in perifusion and intraluminal fluids was determined at one hour after initiation of the sustaining perifusion, and the percentage of radioactivity of 3H-androgen and 14C-PEG appearing in the intraluminal fluid to that in the peritubular fluid was determined. Proluminal movement of 3H-androgens into the caput epididymal tubules in the control rats was 323.4±73.2%. This value was significantly reduced to 121.8±13% by addition of cycloheximide to the perifusion fluid (p<O.O1). Transepithelial movement of 3H-androgen in the caput epididymis was significantly decreased to 86.6±5.3% by exposure of the epididymal tubules to nocodazole (p<O.O1). Inhibition of protein synthesis and disassembly of microtubules in the epididymal epithelial cells completely eliminated antigrade proluminal movement of 3H-androgen into the tubules. Study of the incorporation of 35S-Methionine into epididymal tissue protein revealed significant reduction of the quantity of radiolabeled proteins in the perifused tissue with fluid containing cycloheximide (p<O.O1). These results suggest that antigrade proluminal androgen movement may be related to the synthesis of intracellular cytoplasmic proteins.
内容記述タイプ Abstract
出版者
出版者 Nagoya University School of Medicine
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
ID登録
ID登録 10.18999/nagjms.57.3-4.119
ID登録タイプ JaLC
関連情報
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5734/5734.html
ISSN(print)
収録物識別子タイプ ISSN
収録物識別子 0027-7622
ISSN(Online)
収録物識別子タイプ ISSN
収録物識別子 2186-3326
書誌情報 Nagoya Journal of Medical Science

巻 57, 号 3-4, p. 119-126, 発行日 1994-12-26
著者版フラグ
値 publisher
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