WEKO3
アイテム
EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS
https://doi.org/10.18999/nagjms.57.3-4.119
https://doi.org/10.18999/nagjms.57.3-4.119cb47ec8c-492a-4d53-9edd-5bec1507a521
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
v57n34p119_126.pdf (557.6 kB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-03-06 | |||||
| タイトル | ||||||
| タイトル | EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS | |||||
| 言語 | en | |||||
| 著者 |
YAMAMOTO, MASANORI
× YAMAMOTO, MASANORI× HIBI, HATSUKI× TSUJI, YOSHIKAZU× MIYAKE, KOJI |
|||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Androgen | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Epididymis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cytoplasmic receptor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Methionine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cycloheximide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nocodazole | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The effects of protein synthesis inhibition and disassembly of microtubules in the epididymal epithelia on proluminal movement of 3H-androgens were investigated by using in vivo microperifusion of 3H-testosterone and subsequent micropuncture to obtain peritubular and intraluminal fluids of caput epididymal tubules. Cycloheximide (100 μg/ml) was used as protein synthesis inhibitor. Nocodazole (3 μg/ml) was used to depolymerize microtubules in the cell. The perifusion fluid was Minimum Essential Medium containing 26.7 μCi/ml 3H-testosterone and 1.3 μCi/ml 14C-polyethyleneglycol (14C-PEG), or the same fluid supplemented with cycloheximide or nocodazole. Radioactivity of 3H-androgen and 14C-PEG in perifusion and intraluminal fluids was determined at one hour after initiation of the sustaining perifusion, and the percentage of radioactivity of 3H-androgen and 14C-PEG appearing in the intraluminal fluid to that in the peritubular fluid was determined. Proluminal movement of 3H-androgens into the caput epididymal tubules in the control rats was 323.4±73.2%. This value was significantly reduced to 121.8±13% by addition of cycloheximide to the perifusion fluid (p<O.O1). Transepithelial movement of 3H-androgen in the caput epididymis was significantly decreased to 86.6±5.3% by exposure of the epididymal tubules to nocodazole (p<O.O1). Inhibition of protein synthesis and disassembly of microtubules in the epididymal epithelial cells completely eliminated antigrade proluminal movement of 3H-androgen into the tubules. Study of the incorporation of 35S-Methionine into epididymal tissue protein revealed significant reduction of the quantity of radiolabeled proteins in the perifused tissue with fluid containing cycloheximide (p<O.O1). These results suggest that antigrade proluminal androgen movement may be related to the synthesis of intracellular cytoplasmic proteins. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Nagoya University School of Medicine | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| ID登録 | ||||||
| ID登録 | 10.18999/nagjms.57.3-4.119 | |||||
| ID登録タイプ | JaLC | |||||
| 関連情報 | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5734/5734.html | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0027-7622 | |||||
| ISSN（Online） | ||||||
| 収録物識別子タイプ | EISSN | |||||
| 収録物識別子 | 2186-3326 | |||||
| 書誌情報 |
en : Nagoya Journal of Medical Science 巻 57, 号 3-4, p. 119-126, 発行日 1994-12-26 |
|||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
