{"_buckets": {"deposit": "c7ff55df-d88b-435f-a841-49a1070f9c27"}, "_deposit": {"id": "14168", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "14168"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00014168"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1994-12-26", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3-4", "bibliographicPageEnd": "126", "bibliographicPageStart": "119", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The effects of protein synthesis inhibition and disassembly of microtubules in the epididymal epithelia on proluminal movement of 3H-androgens were investigated by using in vivo microperifusion of 3H-testosterone and subsequent micropuncture to obtain peritubular and intraluminal fluids of caput epididymal tubules. Cycloheximide (100 \u03bcg/ml) was used as protein synthesis inhibitor. Nocodazole (3 \u03bcg/ml) was used to depolymerize microtubules in the cell. The perifusion fluid was Minimum Essential Medium containing 26.7 \u03bcCi/ml 3H-testosterone and 1.3 \u03bcCi/ml 14C-polyethyleneglycol (14C-PEG), or the same fluid supplemented with cycloheximide or nocodazole. Radioactivity of 3H-androgen and 14C-PEG in perifusion and intraluminal fluids was determined at one hour after initiation of the sustaining perifusion, and the percentage of radioactivity of 3H-androgen and 14C-PEG appearing in the intraluminal fluid to that in the peritubular fluid was determined. Proluminal movement of 3H-androgens into the caput epididymal tubules in the control rats was 323.4\u00b173.2%. This value was significantly reduced to 121.8\u00b113% by addition of cycloheximide to the perifusion fluid (p\u003cO.O1). Transepithelial movement of 3H-androgen in the caput epididymis was significantly decreased to 86.6\u00b15.3% by exposure of the epididymal tubules to nocodazole (p\u003cO.O1). Inhibition of protein synthesis and disassembly of microtubules in the epididymal epithelial cells completely eliminated antigrade proluminal movement of 3H-androgen into the tubules. Study of the incorporation of 35S-Methionine into epididymal tissue protein revealed significant reduction of the quantity of radiolabeled proteins in the perifused tissue with fluid containing cycloheximide (p\u003cO.O1). These results suggest that antigrade proluminal androgen movement may be related to the synthesis of intracellular cytoplasmic proteins.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.57.3-4.119", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine"}]}, "item_9_relation_43": {"attribute_name": "\u7570\u7248\u3067\u3042\u308b", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/5734/5734.html", "subitem_relation_type_select": "URI"}}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN\uff08Online\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "ISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "YAMAMOTO, MASANORI"}], "nameIdentifiers": [{"nameIdentifier": "98926", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIBI, HATSUKI"}], "nameIdentifiers": [{"nameIdentifier": "98927", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TSUJI, YOSHIKAZU"}], "nameIdentifiers": [{"nameIdentifier": "98928", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MIYAKE, KOJI"}], "nameIdentifiers": [{"nameIdentifier": "98929", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "v57n34p119_126.pdf", "filesize": [{"value": "557.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 557600.0, "url": {"label": "v57n34p119_126.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/14168/files/v57n34p119_126.pdf"}, "version_id": "f31f5f1d-3c49-4de2-b971-37a0a4f99217"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Androgen", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Epididymis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cytoplasmic receptor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Methionine", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cycloheximide", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Nocodazole", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/614"], "permalink_uri": "https://doi.org/10.18999/nagjms.57.3-4.119", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2012-03-06"}, "publish_date": "2012-03-06", "publish_status": "0", "recid": "14168", "relation": {}, "relation_version_is_last": true, "title": ["EFFECTS OF PROTEIN SYNTHESIS INHIBITOR AND ANTIMICROTUBULAR AGENT ON TRANSEPITHELIAL MOVEMENT OF 3H-ANDROGENS IN THE RAT CAPUT EPIDIDYMIS"], "weko_shared_id": null}