Item type |
itemtype_ver1(1) |
公開日 |
2022-03-08 |
タイトル |
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タイトル |
A Pradimicin-Based Staining Dye for Glycoprotein Detection |
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言語 |
en |
著者 |
Nakagawa, Yu
Kakihara, Shintaro
Tsuzuki, Kazue
Ojika, Makoto
Igarashi, Yasuhiro
Ito, Yukishige
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アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
権利 |
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言語 |
en |
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権利情報 |
This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Journal of Natural Products], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-A6KFIPPCDMPP5SRZ7UST].” |
内容記述 |
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内容記述 |
Pradimicin A (PRM-A) and related compounds constitute an exceptional family of natural pigments that show Ca^2+-dependent recognition of d-mannose (Man). Although these compounds hold great promise as research tools in glycobiology, their practical application has been severely limited by their inherent tendency to form water-insoluble aggregates. Here, we demonstrate that the 2-hydroxyethylamide derivative (PRM-EA) of PRM-A shows little aggregation in neutral aqueous media and retains binding specificity for Man. We also show that PRM-EA stains glycoproteins in dot blot assays, whereas PRM-A fails to do so, owing to severe aggregation. Significantly, PRM-EA is sensitive to glycoproteins carrying high mannose-type and hybrid-type N-linked glycans, but not to those carrying complex-type N-linked glycans. Such staining selectivity has never been observed in conventional dyes, suggesting that PRM-EA could serve as a unique staining agent for the selective detection of glycoproteins with terminal Man residues. |
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言語 |
en |
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内容記述タイプ |
Abstract |
出版者 |
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言語 |
en |
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出版者 |
ACS Publications |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1021/acs.jnatprod.1c00506 |
収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0163-3864 |
書誌情報 |
en : Journal of Natural Products
巻 84,
号 9,
p. 2496-2501,
発行日 2021-09-24
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ファイル公開日 |
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日付 |
2022-09-24 |
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日付タイプ |
Available |