{"_buckets": {"deposit": "cbac292e-d423-4533-a537-5dd31d5e2e1d"}, "_deposit": {"created_by": 17, "id": "2007900", "owner": "17", "owners": [17], "owners_ext": {"displayname": "repository", "username": "repository"}, "pid": {"revision_id": 0, "type": "depid", "value": "2007900"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:02007900"}, "author_link": [], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_1629683748249": {"attribute_name": "日付", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2024-05-01", "subitem_date_issued_type": "Available"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2023-05", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "154443", "bibliographicVolumeNumber": "245", "bibliographic_titles": [{"bibliographic_title": "Pathology - Research and Practice", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "Osteosarcoma, the most common primary malignant bone tumor, is defined by the formation of neoplastic osteoid and/or bone. This sarcoma is a highly heterogeneous disease with a wide range of patient outcomes. CD109 is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed in various types of malignant tumors. We previously reported that CD109 is expressed in osteoblasts and osteoclasts in normal human tissues and plays a role in bone metabolism in vivo. While CD109 has been shown to promote various carcinomas through the downregulation of TGF-β signaling, the role and mechanism of CD109 in sarcomas remain largely unknown. In this study, we investigated the molecular function of CD109 in sarcomas using osteosarcoma cell lines and tissue. Semi-quantitative immunohistochemical analysis using human osteosarcoma tissue revealed a significantly worse prognosis in the CD109-high group compared with the CD109-low group. We found no association between CD109 expression and TGF-β signaling in osteosarcoma cells. However, enhancement of SMAD1/5/9 phosphorylation was observed in CD109 knockdown cells under bone morphogenetic protein-2 (BMP-2) stimulation. We also performed immunohistochemical analysis for phospho-SMAD1/5/9 using human osteosarcoma tissue and found a negative correlation between CD109 expression and SMAD1/5/9 phosphorylation. In vitro wound healing assay showed that osteosarcoma cell migration was significantly attenuated in CD109-knockdown cells compared with control cells in the presence of BMP. These results suggest that CD109 is a poor prognostic factor in osteosarcoma and affects tumor cell migration via BMP signaling.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier", "subitem_publisher_language": "en"}]}, "item_9_relation_43": {"attribute_name": "関連情報", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/j.prp.2023.154443", "subitem_relation_type_select": "DOI"}}]}, "item_9_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2023. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/", "subitem_rights_language": "en"}]}, "item_9_source_id_7": {"attribute_name": "収録物識別子", "attribute_value_mlt": [{"subitem_source_identifier": "0344-0338", "subitem_source_identifier_type": "PISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "embargoed access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_f1cf"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Mori, Natsumi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Esaki, Nobutoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shimoyama, Yoshie", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Shiraki, Yukihiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Asai, Naoya", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Sakai, Tomohisa", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nishida, Yoshihiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Takahashi, Masahide", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Enomoto, Atsushi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mii, Shinji", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2024-05-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "01_20230330_bone-tumor.pdf", "filesize": [{"value": "65.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "application/pdf", "size": 65599999.99999999, "url": {"objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/2007900/files/01_20230330_bone-tumor.pdf"}, "version_id": "fb2e30fe-b9f2-4678-a66b-ffb90e624538"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2024-05-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "02_20230328_bone-tumor_supple.pdf", "filesize": [{"value": "32.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "application/pdf", "size": 32500000.0, "url": {"objectType": "dataset", "url": "https://nagoya.repo.nii.ac.jp/record/2007900/files/02_20230328_bone-tumor_supple.pdf"}, "version_id": "f58bb966-b0b2-42e4-9982-de7292a2f3f3"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "CD109", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Osteosarcoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Prognostic marker", "subitem_subject_scheme": "Other"}, {"subitem_subject": "BMP", "subitem_subject_scheme": "Other"}, {"subitem_subject": "SMAD", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling", "subitem_title_language": "en"}]}, "item_type_id": "40001", "owner": "17", "path": ["501"], "permalink_uri": "http://hdl.handle.net/2237/0002007900", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2023-10-18"}, "publish_date": "2023-10-18", "publish_status": "0", "recid": "2007900", "relation": {}, "relation_version_is_last": true, "title": ["Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling"], "weko_shared_id": -1}
Significance of expression of CD109 in osteosarcoma and its involvement in tumor progression via BMP signaling
http://hdl.handle.net/2237/0002007900
http://hdl.handle.net/2237/0002007900