{"_buckets": {"deposit": "ba042d97-c9d9-45d7-8f58-b8dba972b3ce"}, "_deposit": {"id": "24930", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24930"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024930"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "670", "bibliographicPageStart": "661", "bibliographicVolumeNumber": "32", "bibliographic_titles": [{"bibliographic_title": "Neurotoxicity Research"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Previous studies showed that overexposure to manganese causes parkinsonism, a disorder of dopaminergic neurons. Previous studies also showed that activity of c-RET kinase controls dopamine production through regulation of tyrosine hydroxylase (TH) expression, suggesting the involvement of c-RET in the development of parkinsonism. To our knowledge, however, there is no report showing a correlation between manganese-mediated parkinsonism and c-RET. In this study, we examined the effect of manganese on the expression and/or activation levels of c-RET and TH in human TH-expressing cells (TGW cells). We first found that treatment with 30 and 100 \u03bcM manganese resulted in reduction of c-RET transcript level and degradation of c-RET protein through promotion of ubiquitination. We then examined the biological significance of manganese-mediated decrease of c-RET protein expression. Decreased TH expression with decreased c-RET kinase activity was observed in c-RET protein-depleted TGW cells by treatment with manganese (30 \u03bcM) as well as by c-RET siRNA transfection. Since TH protein has been shown to be involved in the dopamine-producing pathway in previous studies, our results indicate the possibility that manganese-mediated reduction of TH expression and phosphorylation via decreased expression of c-RET protein in neural cells is involved in parkinsonism induced by manganese.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1007/s12640-017-9783-0"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27152"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Springer"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "The final publication is available at Springer via http://doi.org/10.1007/s12640-017-9783-0"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1029-8428", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kumasaka, Mayuko Y."}], "nameIdentifiers": [{"nameIdentifier": "74266", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yajima, Ichiro"}], "nameIdentifiers": [{"nameIdentifier": "74267", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohgami, Nobutaka"}], "nameIdentifiers": [{"nameIdentifier": "74268", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ninomiya, Hiromasa"}], "nameIdentifiers": [{"nameIdentifier": "74269", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iida, Machiko"}], "nameIdentifiers": [{"nameIdentifier": "74270", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Li, Xiang"}], "nameIdentifiers": [{"nameIdentifier": "74271", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Oshino, Reina"}], "nameIdentifiers": [{"nameIdentifier": "74272", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanihata, Hiroko"}], "nameIdentifiers": [{"nameIdentifier": "74273", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshinaga, Masafumi"}], "nameIdentifiers": [{"nameIdentifier": "74274", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kato, Masashi"}], "nameIdentifiers": [{"nameIdentifier": "74275", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-11-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Kumasaka_et_al.pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2700000.0, "url": {"label": "Kumasaka_et_al.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2018/11/01", "url": "https://nagoya.repo.nii.ac.jp/record/24930/files/Kumasaka_et_al.pdf"}, "version_id": "864bf2c5-3417-4d47-a303-35b37892b2f0"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Manganese", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Metals", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Protein expression", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RET", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Tyrosine kinase", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro"}]}, "item_type_id": "10", "owner": "1", "path": ["499/500/501"], "permalink_uri": "http://hdl.handle.net/2237/27152", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-12-07"}, "publish_date": "2017-12-07", "publish_status": "0", "recid": "24930", "relation": {}, "relation_version_is_last": true, "title": ["Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro"], "weko_shared_id": null}