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Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro
http://hdl.handle.net/2237/27152
http://hdl.handle.net/2237/2715232eb0885-593d-46cc-84fd-2719eb471117
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Kumasaka_et_al.pdf ファイル公開日:2018/11/01 (2.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-12-07 | |||||
| タイトル | ||||||
| タイトル | Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro | |||||
| 言語 | en | |||||
| 著者 |
Kumasaka, Mayuko Y.
× Kumasaka, Mayuko Y.× Yajima, Ichiro× Ohgami, Nobutaka× Ninomiya, Hiromasa× Iida, Machiko× Li, Xiang× Oshino, Reina× Tanihata, Hiroko× Yoshinaga, Masafumi× Kato, Masashi |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | The final publication is available at Springer via http://doi.org/10.1007/s12640-017-9783-0 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Manganese | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Metals | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Protein expression | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | RET | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Tyrosine kinase | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Previous studies showed that overexposure to manganese causes parkinsonism, a disorder of dopaminergic neurons. Previous studies also showed that activity of c-RET kinase controls dopamine production through regulation of tyrosine hydroxylase (TH) expression, suggesting the involvement of c-RET in the development of parkinsonism. To our knowledge, however, there is no report showing a correlation between manganese-mediated parkinsonism and c-RET. In this study, we examined the effect of manganese on the expression and/or activation levels of c-RET and TH in human TH-expressing cells (TGW cells). We first found that treatment with 30 and 100 μM manganese resulted in reduction of c-RET transcript level and degradation of c-RET protein through promotion of ubiquitination. We then examined the biological significance of manganese-mediated decrease of c-RET protein expression. Decreased TH expression with decreased c-RET kinase activity was observed in c-RET protein-depleted TGW cells by treatment with manganese (30 μM) as well as by c-RET siRNA transfection. Since TH protein has been shown to be involved in the dopamine-producing pathway in previous studies, our results indicate the possibility that manganese-mediated reduction of TH expression and phosphorylation via decreased expression of c-RET protein in neural cells is involved in parkinsonism induced by manganese. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Springer | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s12640-017-9783-0 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 1029-8428 | |||||
| 書誌情報 |
en : Neurotoxicity Research 巻 32, 号 4, p. 661-670, 発行日 2017-11 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://doi.org/10.1007/s12640-017-9783-0 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/27152 | |||||
| 識別子タイプ | HDL | |||||
