ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "77f17107-2854-4325-b1f4-eff52277d2fc"}, "_deposit": {"id": "26123", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "26123"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00026123", "sets": ["696"]}, "author_link": ["78364", "78365", "78366", "78367", "78368"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-02-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "497", "bibliographicPageStart": "494", "bibliographicVolumeNumber": "57", "bibliographic_titles": [{"bibliographic_title": "Biochemistry", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The light oxygen voltage-sensing (LOV) domain plays a crucial role in blue light (BL) sensing in plants and microorganisms. LOV domains are usually associated with the effector domains and regulate the activities of effector domains in a BL-dependent manner. Photozipper (PZ) is monomeric in the dark state. BL induces reversible dimerization of PZ and subsequently increases its affinity for the target DNA sequence. In this study, we report the analyses of PZ by pulsed electron–electron double resonance (PELDOR). The neutral flavin radical was formed by BL illumination in the presence of dithiothreitol in the LOV-C254S (without the bZIP domain) and PZ-C254S mutants, where the cysteine residue responsible for adduct formation was replaced with serine. The magnetic dipole interactions of 3 MHz between the neutral radicals were detected in both LOV-C254S and PZ-C254S, indicating that these mutants are dimeric in the radical state. The PELDOR simulation showed that the distance between the radical pair is close to that estimated from the dimeric crystal structure in the “light state” [Heintz, U., and Schlichting, I. (2016) eLife 5, e11860], suggesting that in the radical state, LOV domains in PZ-C254S form a dimer similar to that of LOV-C254S, which lacks the bZIP domain.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "ファイル公開:2019/02/06", "subitem_description_language": "ja", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "ACS Publications", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1021/acs.biochem.7b01045", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Biochemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [http://pubs.acs.org/articlesonrequest/AOR-3F5DaaWT3x2Ir9KDijgv].” ", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-2960", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ozeki, Kohei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "78364", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tsukuno, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "78365", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nagashima, Hiroki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "78366", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hisatomi, Osamu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "78367", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mino, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "78368", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-02-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "manuscriptPELDORforRep.pdf", "filesize": [{"value": "410.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 410800.0, "url": {"label": "manuscriptPELDORforRep", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/26123/files/manuscriptPELDORforRep.pdf"}, "version_id": "70715470-88aa-4c80-b6a0-93ef7642de68"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["696"], "permalink_uri": "http://hdl.handle.net/2237/00028327", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-07-02"}, "publish_date": "2018-07-02", "publish_status": "0", "recid": "26123", "relation": {}, "relation_version_is_last": true, "title": ["Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State"], "weko_shared_id": -1}
  1. B100 理学部/理学研究科
  2. B100a 雑誌掲載論文
  3. 学術雑誌

Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State

http://hdl.handle.net/2237/00028327
http://hdl.handle.net/2237/00028327
e1724cd2-253a-4a55-b4c8-ffd35e9c5200
名前 / ファイル ライセンス アクション
manuscriptPELDORforRep.pdf manuscriptPELDORforRep (410.8 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2018-07-02
タイトル
タイトル Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State
言語 en
著者 Ozeki, Kohei

× Ozeki, Kohei

WEKO 78364

en Ozeki, Kohei

Search repository
Tsukuno, Hiroyuki

× Tsukuno, Hiroyuki

WEKO 78365

en Tsukuno, Hiroyuki

Search repository
Nagashima, Hiroki

× Nagashima, Hiroki

WEKO 78366

en Nagashima, Hiroki

Search repository
Hisatomi, Osamu

× Hisatomi, Osamu

WEKO 78367

en Hisatomi, Osamu

Search repository
Mino, Hiroyuki

× Mino, Hiroyuki

WEKO 78368

en Mino, Hiroyuki

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 “This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Biochemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [http://pubs.acs.org/articlesonrequest/AOR-3F5DaaWT3x2Ir9KDijgv].”
抄録
内容記述 The light oxygen voltage-sensing (LOV) domain plays a crucial role in blue light (BL) sensing in plants and microorganisms. LOV domains are usually associated with the effector domains and regulate the activities of effector domains in a BL-dependent manner. Photozipper (PZ) is monomeric in the dark state. BL induces reversible dimerization of PZ and subsequently increases its affinity for the target DNA sequence. In this study, we report the analyses of PZ by pulsed electron–electron double resonance (PELDOR). The neutral flavin radical was formed by BL illumination in the presence of dithiothreitol in the LOV-C254S (without the bZIP domain) and PZ-C254S mutants, where the cysteine residue responsible for adduct formation was replaced with serine. The magnetic dipole interactions of 3 MHz between the neutral radicals were detected in both LOV-C254S and PZ-C254S, indicating that these mutants are dimeric in the radical state. The PELDOR simulation showed that the distance between the radical pair is close to that estimated from the dimeric crystal structure in the “light state” [Heintz, U., and Schlichting, I. (2016) eLife 5, e11860], suggesting that in the radical state, LOV domains in PZ-C254S form a dimer similar to that of LOV-C254S, which lacks the bZIP domain.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2019/02/06
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 ACS Publications
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1021/acs.biochem.7b01045
ISSN
収録物識別子タイプ PISSN
収録物識別子 0006-2960
書誌情報 en : Biochemistry

巻 57, 号 5, p. 494-497, 発行日 2018-02-06
著者版フラグ
値 author
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 12:29:14.906841
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3