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Identification of plasma antibody epitopes and gene abnormalities in Japanese hemophilia a patients with factor VIII inhibitor
https://doi.org/10.18999/nagjms.63.1-2.25
https://doi.org/10.18999/nagjms.63.1-2.2591720df1-c12a-4753-880f-3f6a7df2344c
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2006-02-03 | |||||
タイトル | ||||||
タイトル | Identification of plasma antibody epitopes and gene abnormalities in Japanese hemophilia a patients with factor VIII inhibitor | |||||
著者 |
Sugihara, Takuro
× Sugihara, Takuro× Takahashi, Isao× Kojima, Tetsuhito× Okamoto, Yoshihiro× Yamamoto, Koji× Kamiya, Tadashi× Matsushita, Tadashi× Saito, Hidehiko |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Factor VIII | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hemophilia A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Factor VIII inhibitor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Southern blotting | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene inversion | |||||
抄録 | ||||||
内容記述 | Eleven Japanese hemophilia A patients with anti-factor VIII (FVIII)a) inhibitors were studied to localize both their inhibitory antibody epitopes and their genotypes. The inhibitor epitopes were studied in nine severe hemophilia A patients by means of a scanning method using the oligopeptide panel covering the FVIII polypeptides without the B domain. The 107 15 mer-peptides were synthesized on solid-phase pins and analyzed for their reactivity with diluted patient plasma. As indicated previously, a series of peptides corresponding to the A2 and C2 domains were recognized by plasma antibodies from 2 patients and 4 patients, respectively. In contrast, all the antibodies bound to several epitopes in the A3 domain, while an epitope 1809-1821 covering the putative factor IX binding site was found in 3 patients. Southern blotting analysis showed that 8 out of 11 patients had either gene deletions or inversions of the FVIII gene, indicating a higher proportion of gross gene alterations in inhibitor-positive hemophilia A patients. However, the correlation of gene abnormality type with epitope location was not fully established. | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
出版者 | Nagoya University School of Medicine | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.18999/nagjms.63.1-2.25 | |||||
ID登録タイプ | JaLC | |||||
書誌情報 |
Nagoya Journal of Medical Science 巻 63, 号 1-2, p. 25-39, 発行日 2000-05 |
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フォーマット | ||||||
application/pdf | ||||||
著者版フラグ | ||||||
値 | publisher | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/5351 | |||||
識別子タイプ | HDL |