{"_buckets": {"deposit": "370d8398-69da-4acf-bd97-f14808e502e6"}, "_deposit": {"id": "3977", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "3977"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00003977"}, "item_9_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3-4", "bibliographicPageEnd": "108", "bibliographicPageStart": "101", "bibliographicVolumeNumber": "67", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science"}]}]}, "item_9_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Malignant transformation of cells is frequently associated with an augmented production of hyaluronan and the subsequent formation of a hyaluronan-matrix. In v-Src-transformed cells, hyaluronan directly activate cell motility in a tumor-specifi c manner. Despite its importance, the mechanism by which v-Src activates hyaluronan production remains unclear. Here we report that multiple signaling pathways are required for the augmented production of hyaluronan. Either the expression of a dominant negative Ras or the treatment of cells with manumycin A, a Ras farnesyltransferase inhibitor, was able to suppress hyaluronan production. In contrast, expression of MEK1EE, a constitutive form of MEK1, activated both hyaluronan synthase expression and hyaluronan production. AG-490, a Jak-2 inhibitor, or LY294002, a PI3K inhibitor, similarly suppressed the augmented production of hyarulonan. Taken together, our results suggest the involvement of multiple signaling pathways, including Ras-dependent and independent ones, in augmented hyaluronan production by v-Src.", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/5410"}]}, "item_9_identifier_registration": {"attribute_name": "ID\u767b\u9332", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.67.3-4.101", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of  Medicine"}]}, "item_9_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_text_14": {"attribute_name": "\u30d5\u30a9\u30fc\u30de\u30c3\u30c8", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Naito, Yuko"}], "nameIdentifiers": [{"nameIdentifier": "10202", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Noriko"}], "nameIdentifiers": [{"nameIdentifier": "10203", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Huang, Pengyu"}], "nameIdentifiers": [{"nameIdentifier": "10204", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hasegawa, Hitoki"}], "nameIdentifiers": [{"nameIdentifier": "10205", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sohara, Yasuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "10206", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iwamoto, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "10207", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hamaguchi, Michinari"}], "nameIdentifiers": [{"nameIdentifier": "10208", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "p101NAITO.pdf", "filesize": [{"value": "322.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 322300.0, "url": {"label": "p101NAITO.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/3977/files/p101NAITO.pdf"}, "version_id": "55f9a774-85cb-4c57-b2e6-ed13f0938500"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Hyaluronan", "subitem_subject_scheme": "Other"}, {"subitem_subject": "v-Src", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Signaling", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ras", "subitem_subject_scheme": "Other"}, {"subitem_subject": "MEK1", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Requirement of Multiple Signaling Pathways for the Augmented Production of Hyaluronan by V-SRC", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Requirement of Multiple Signaling Pathways for the Augmented Production of Hyaluronan by V-SRC"}]}, "item_type_id": "9", "owner": "1", "path": ["499/508/509/520"], "permalink_uri": "https://doi.org/10.18999/nagjms.67.3-4.101", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2006-02-10"}, "publish_date": "2006-02-10", "publish_status": "0", "recid": "3977", "relation": {}, "relation_version_is_last": true, "title": ["Requirement of Multiple Signaling Pathways for the Augmented Production of Hyaluronan by V-SRC"], "weko_shared_id": null}